Jo-1 Antibody (Jo1/HRS) ELISA Kit
Reactivity: Human
Colorimetric
Competition ELISA
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- Target
- Jo-1 Antibody (Jo1/HRS)
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Reactivity
- Human
- Detection Method
- Colorimetric
- Method Type
- Competition ELISA
- Application
- ELISA
- Analytical Method
- Quantitative
- Characteristics
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ELISA kit for the detection of Jo1 in the research laboratory
Alternative Names: Jo1 ELISA kit
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- Application Notes
- Optimal conditions to be determined by end-user
- Plate
- Pre-coated
- Assay Procedure
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Highly purified Jo1 is bound to microwells. Antibodies to this antigen, if present in diluted serum, bind in the microwells. Washing of the microwells removes unbound serum antibodies. Horseradish peroxidase (HRP) conjugated antihuman IgG immunologically bind to the bound patient antibodies forming a conjugate/antibody/antigen complex. Washing of the microwells removes unbound conjugate. An enzyme substrate in the presence of bound conjugate hydrolyzes to form a blue color. The addition of an acid stops the reaction forming a yellow endproduct. The intensity of this yellow color is measured photometrically at 450 nm. The amount of colour is directly proportional to the concentration of IgG antibodies present in the original sample.
- Restrictions
- For Research Use only
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- Storage
- 4 °C
- Storage Comment
- Store at 2-8 °C.
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- Target
- Jo-1 Antibody (Jo1/HRS)
- Target Type
- Antibody
- Background
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Anti-Jo1 antibodies, are associated with a more abrupt onset of fever, cracked hands, Raynaud's phenomenon, interstitial lung disease, arthritis, and a poor response to therapy. Anti-Jo1 antibodies are a type of antinuclear antibodies. HistidinetRNA ligase is the target of antiJo1 antibodies.
Synonyms: Jo1 ELISA kit.
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