Anti-Parietal Cell ELISA Kit

Catalog No. ABIN930717

This Human ELISA Kit is a Colorimetric ELISA Kit designed to quantify Human .

Quick Overview for Anti-Parietal Cell ELISA Kit (ABIN930717)

Target: Anti-Parietal Cell

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Product Details

Analytical Method: Quantitative

Characteristics: ELISA kit for the detection of Parietal Cell in the research laboratory
Alternative Names: Parietal cell ELISA kit

Purification: Ascites, ammnonium sulphate purified

Application Details

Application Notes: Optimal conditions to be determined by end-user

Plate: Pre-coated

Assay Procedure:

AntiParietal Cell (H+/K+ATPase) is an indirect solid phase enzyme immunometric assay (ELISA). It is designed for the quantitative measurement of IgG class autoantibodies directed against the a and bsubunits of the Parietal Cell H+/K+ATPase. Preparations of highly purified pig Parietal Cell H+/K+ATPase are coated on microplates. The microplates can be divided into 12 modules of 8 wells each or can be used completely for 96 determinations. Each well can be separated from the module (

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: Store at 2-8 °C.

Target Details

Target: Anti-Parietal Cell

Target Type: Antibody

Background: Autoantibodies against the H/K ATPase can be detected in 80-90 % of pernicious anemia patients, by indirect immunofluorescence and they are also detected in 20 % of the healthy adult population. ELISA test systems show a sensitivity of about 80 % and specificity of about 90 %. There is an age related increase in the presence of parietal cell autoantibodies in the adult population. A study of the relationship between parietal cell autoantibody and gastric mucosal morphology, indicates these parietal cell positive individuals in a random population may indeed have early type A gastritis. Higher prevalence rates (20-30 %) of parietal cell autoantibodies have been noted in patients with autoimmune endocrine disorders such as thyrotoxicosis, Hashimoto's thyroiditis and insulin-dependent diabetes. Histological examinations of gastric biopsies reveals that the majority of parietal cell autoantibody positive individuals also have a type A gastric lesion.
Synonyms: Parietal cell ELISA kit.