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Anti-Prothrombin IgG/IgM ELISA Kit

Reactivity: Human Colorimetric Sandwich ELISA
Catalog No. ABIN930729
  • Target
    Anti-Prothrombin IgG/IgM
    Reactivity
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Application
    ELISA
    Analytical Method
    Quantitative
    Characteristics
    ELISA kit for the detection of Prothrombin IgG/IgM in the research laboratory
    Alternative Names: Prothrombin IgG/IgM ELISA kit
  • Application Notes
    Optimal conditions to be determined by end-user
    Plate
    Pre-coated
    Assay Procedure

    The AntiProthrombin IgG/IgM is an indirect solid phase enzyme immunometric assay (ELISA). It is designed for the quantitative measurement of IgG or IgM class autoantibodies directed against Prothrombin. The microplate is coated with highly purified prothrombin. The microplate can be divided into 12 modules of 8 wells each or can be used completely for 96 determinations. Each well can be separated from the module (

    Restrictions
    For Research Use only
  • Storage
    4 °C
    Storage Comment
    Store at 2-8 °C.
  • Target
    Anti-Prothrombin IgG/IgM
    Target Type
    Antibody
    Background
    Prothrombin (factor II), exerts a procoagulant activity via a prothrombinase complex, triggering fibrinogen conversion to fibrin. Autoantibodies can inhibit the formation of the thrombokinase complex. This complex is formed by the binding of prothrombin to phospholipids on the damaged cell membrane where prothrombin, then, is enzymatically transformed into thrombin. Thrombin, itself, causes blood coagulation. Antibodies directed against coagulation factors, like prothrombin, are pathogenic antibodies, that circulate in the blood stream. They directly inhibit the coagulation factors, and hence prolong the coagulation time. These autoantibodies are also found in association with autoimmune diseases. The presence of antibodies to prothrombin is associated with thrombosis in patients with SLE and APS. And it was found that high autoantibody levels against prothrombin imply a risk of deep venous thrombosis and pulmonary embolism and could be involved in the development of the thrombotic processes. Furthermore, it was demonstrated that elevated levels of antibodies to prothrombin predict a risk of myocardial infarction.
    Synonyms: Prothrombin IgG/IgM ELISA kit.
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