Anti-Prothrombin IgG/IgM ELISA Kit

Catalog No. ABIN930729

This Human ELISA Kit is a Colorimetric ELISA Kit designed to quantify Human .

Quick Overview for Anti-Prothrombin IgG/IgM ELISA Kit (ABIN930729)

Target: Anti-Prothrombin IgG/IgM

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Product Details

Analytical Method: Quantitative

Characteristics: ELISA kit for the detection of Prothrombin IgG/IgM in the research laboratory
Alternative Names: Prothrombin IgG/IgM ELISA kit

Application Details

Application Notes: Optimal conditions to be determined by end-user

Plate: Pre-coated

Assay Procedure:

The AntiProthrombin IgG/IgM is an indirect solid phase enzyme immunometric assay (ELISA). It is designed for the quantitative measurement of IgG or IgM class autoantibodies directed against Prothrombin. The microplate is coated with highly purified prothrombin. The microplate can be divided into 12 modules of 8 wells each or can be used completely for 96 determinations. Each well can be separated from the module (

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: Store at 2-8 °C.

Target Details

Target: Anti-Prothrombin IgG/IgM

Target Type: Antibody

Background: Prothrombin (factor II), exerts a procoagulant activity via a prothrombinase complex, triggering fibrinogen conversion to fibrin. Autoantibodies can inhibit the formation of the thrombokinase complex. This complex is formed by the binding of prothrombin to phospholipids on the damaged cell membrane where prothrombin, then, is enzymatically transformed into thrombin. Thrombin, itself, causes blood coagulation. Antibodies directed against coagulation factors, like prothrombin, are pathogenic antibodies, that circulate in the blood stream. They directly inhibit the coagulation factors, and hence prolong the coagulation time. These autoantibodies are also found in association with autoimmune diseases. The presence of antibodies to prothrombin is associated with thrombosis in patients with SLE and APS. And it was found that high autoantibody levels against prothrombin imply a risk of deep venous thrombosis and pulmonary embolism and could be involved in the development of the thrombotic processes. Furthermore, it was demonstrated that elevated levels of antibodies to prothrombin predict a risk of myocardial infarction.
Synonyms: Prothrombin IgG/IgM ELISA kit.