Anti-ASCA IgA/IgG ELISA Kit
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- Target
- Anti-ASCA IgA/IgG
- Reactivity
- Human
- Detection Method
- Colorimetric
- Method Type
- Competition ELISA
- Application
- ELISA
- Analytical Method
- Quantitative
- Characteristics
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ELISA kit for the detection of ASCA IgA/IgG in the research laboratory
Alternative Names: Anti-Saccharomyces cerevisiae antibodies ELISA kit
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- Application Notes
- Optimal conditions to be determined by end-user
- Plate
- Pre-coated
- Assay Procedure
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Highly purified mannan from Saccharomyces cerevisiae is bound to microwells. Antibodies to this antigen, if present in diluted serum, bind in the microwells. Washing of the microwells removes unbound serum antibodies. Horseradish peroxidase (HRP) conjugated antihuman IgG or IgA immunologically bind to the bound patient antibodies forming a conjugate/antibody/antigen complex. Washing of the microwells removes unbound conjugate. An enzyme substrate in the presence of bound conjugate hydrolyzes to form a blue color. The addition of an acid stops the reaction forming a yellow endproduct. The intensity of this yellow color is measured photometrically at 450 nm. The amount of colour is directly proportional to the concentration of IgG resp. IgA antibodies present in the original sample.
- Restrictions
- For Research Use only
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- Storage
- 4 °C
- Storage Comment
- Store at 2-8 °C.
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- Target
- Anti-ASCA IgA/IgG
- Target Type
- Antibody
- Background
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Accurate diagnosis of inflammatory bowel disease (IBD), in particular the differentiation between the two major IBDs ulcerative colitis and Crohn´s disease, is important for treatment and prognosis. Ulcerative colitis is characterized by an inflammation and ulcers in the top layers of the lining of the colon and rectum. Crohn´s disease shows a wide spread inflammation of the gastrointestinal tract with granuloma formation extending deep into the affected tissue. Inflammation in Crohn´s disease is asymmetrical and segmental, with areas of both healthy and diseased tissue, in contrast to ulcerative colitis where inflammation is symmetrical and uninterrupted from the rectum proximally. To differentiate between Crohn´s disease and ulcerative colitis the detection of ANCA (Anti Neutrophil Cytoplasmic Antibody) and ASCA (Anti-Saccharomyces cerevisiae antibody) can be used. ASCA are directed against oligomannosidic epitopes on the cell wall mannan (phosphopeptidomannan) of the yeast Saccharomyces cerevisiae. IgG as well as IgA ASCA show a specificity of 95-100 % for Crohn´s disease. ASCA are strongly associated to Crohn´s disease. Studies showed 0 % positive IgG and 0 % IgA class ASCA in ulcerative colitis whereas in Crohn´s disease a sensitivity of 70 % for IgG and 60 % for IgA class ASCA could be observed. The occurence of atypical ANCA (aANCA) in Crohn´s disease is more infrequent than in ulcerative colitis. The prevalence of ANCA varies from 50 % to 90 % in ulcerative colitis and 10 % to 20 % in Crohn´s disease. The combination of both serological tests makes possible a rapid and noninvasive differential diagnosis between Crohn´s disease and ulcerative colitis.
Synonyms: Anti-Saccharomyces cerevisiae antibodies ELISA kit.
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