Microalbumin ELISA Kit
Reactivity: Human
Competition ELISA
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- Target
- Microalbumin
- Reactivity
- Human
- Method Type
- Competition ELISA
- Application
- ELISA
- Characteristics
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ELISA kit for the detection of MicroAlbumin in the research laboratory
Alternative Names: Albumin ELISA kit
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- Application Notes
- Optimal conditions to be determined by end-user
- Assay Procedure
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MicroAlbumin is a competitive solid phase enzyme immunoassay (ELISA). It is designed for the quantitative measurement of human Albumin in urine. The microplate is coated with highly purified human Albumin. The plate can be divided into 12 modules of 8 wells each or can be used completely for 96 determinations. Each well can beseparated from the module (
- Restrictions
- For Research Use only
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- Storage
- 4 °C
- Storage Comment
- Store at 2-8 °C.
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- Target
- Microalbumin
- Background
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Proteins passing the glomerular basal membrane of the kidney undergo differentiated filtering. The permeability is inversly proportional to the molecular weight (Albumin about 0.6 %, Myoglobulin about 76 %). Nevertheless, only minimal quantities of protein are detectable in urine, because large quantities of protein are reabsorbed by the tubuli. Elevated glomerular protein permeability and high tubular plasma protein elimination can be differentiated by measuring the molecular weight distribution of the eliminated proteins. Albumin has a relative molecular mass of 66,000 Da. It is contained in urine at very low concentrations. In case of a very active glomerular filtering process the albumin secretion can arise without an underlying kidney disease. This situation is called Microalbuminuria. The detection of these small secretion quantities (30 to 150 µg/min or ml) requires very sensitive test systems, i.e. immunological techniques. Physical stress can induce elevated albumin secretion too, without the occurence of a kidney disease. In Diabetes, Albumin secretion is a very important parameter for the evaluation of the kidney function. Urine values higher than 25 µg/mL indicate a detrimental kidney function of insulin-dependent (type I) and noninsulin-dependent (type II) diabetic patients. The determination of Albumin is therefore an important diagnostic tool in diabetic nephropathies.
Synonyms: Albumin ELISA kit.
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