Anti-dsDNA IgG ELISA Kit

Catalog No. ABIN930745

Various Species ELISA Kit Colorimetric assay for quantification of Various Species .

Quick Overview for Anti-dsDNA IgG ELISA Kit (ABIN930745)

Target: Anti-dsDNA IgG

Reactivity: Various Species

Detection Method: Colorimetric

Method Type: Competition ELISA

Application: ELISA

Product Details

Analytical Method: Quantitative

Characteristics: ELISA kit for the detection of dsDNA IgG in the research laboratory
Alternative Names: dsDNA IgG ELISA kit

Application Details

Application Notes: Optimal conditions to be determined by end-user

Plate: Pre-coated

Assay Procedure:

Human recombinant doublestranded DNA (dsDNA) is bound to microwells. Antibodies to this antigen, if present in diluted serum, bind in the microwells. Washing of the microwells removes unbound serum antibodies. Horseradish peroxidase (HRP) conjugated antihuman IgG immunologically bind to the bound patient antibodies forming a conjugate/antibody/antigen complex. Washing of the microwells removes unbound conjugate. An enzyme substrate in the presence of bound conjugate hydrolyzes to form a blue color. The addition of an acid stops the reaction forming a yellow endproduct. The intensity of this yellow color is measured photometrically at 450 nm. The amount of colour is directly proportional to the concentration of IgG antibodies present in the original sample.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: Store at 2-8 °C.

Target Details

Target: Anti-dsDNA IgG

Target Type: Antibody

Background: Deoxyribonucleic acid (DNA) is a nucleic acid that contains the genetic instructions used in the development and functioning of all known living organisms (with the exception of RNA viruses). DNA consists of two long polymers of simple units called nucleotides, with backbones made of sugars and phosphate groups joined by ester bonds. These two strands run in opposite directions to each other and are therefore antiparallel. Attached to each sugar is one of four types of molecules called nucleobases. It is the sequence of these four nucleobases along the backbone that encodes information. This information is read using the genetic code, which specifies the sequence of the amino acids within proteins. The code is read by copying stretches of DNA into the related nucleic acid RNA in a process called transcription.
Synonyms: dsDNA IgG ELISA kit.