DNA Oxidative Damage - Quantitative Kit

Catalog No. ABIN955769

Quick Overview for DNA Oxidative Damage - Quantitative Kit (ABIN955769)

Target: Oxidative DNA Damage

Detection Method: Colorimetric

Product Details

Application Details

Restrictions: For Research Use only

Handling

Target Details

Target: Oxidative DNA Damage

Alternative Name: Oxidative DNA Quantitative

Background: The Oxidative DNA Damage Kit, Quantitative is for the determination of abasic sites in sample DNA, which correspond to colorimetric 96-well microplate assay. ARP standard DNA solutions in this kit are prepared by heat / acid depurination of calf thymus DNA to control the number of abasic sites from 0 to 40 per 1x10 5 bp. These DNA solutions are treated with ARP and purified. The Standard 0 ARP-DNA is prepared using methoxyamine-treated calf thymus DNA without heat/ acid depurination. Since this kit contains centrifugal filtration tubes for the purification of ARP-labeled sample DNA, there is no need to determine the DNA concentration after the ARP reaction. Standard ARP DNA and purified ARP-treated sample DNA are fixed on the 96 well plate with DNA Binding Solution. Then, the number of AP sites in the sample DNA can be determined by the biotin-avidin-peroxidase assay. Since the standard ARP DNA are double GENERAL.Purification of genomic DNA: Several different methods and products are available for the isolation of genomic DNA from samples such as membrane binding method, guanidine/detergent lysis method, and polyelectrolyte precipitation method. Among these methods, the guanidine/detergent lysis method is simple, and it gives highly purified genomic DNA for the ARP-based abasic sites detection.