Quick Overview for TRAP Staining Kit (ABIN955778)
Target
See all CD40 Ligand (CD40LG) Kits-
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Components
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Fixative, 10% Formalin, neutral buffer (Reagent 1): 1 bottle, 50 mL
Tartrate-containing Buffer, 50 mM, pH 5.0 (Reagent 2): 1 bottle, 50 mL
Chromogenic Substrate (Reagent 3): 10 vials, 3 mg/vial
Kit Size: Enough reagents for staining 10 x 96-well plates. -
Material not included
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Adjustable pipettor
Microplate reader or spectrophotometer capable of reading absorbance at 540 nm
Distilled or de-ionized water (dH2O)
Phosphate Buffered Saline (PBS)
37°C incubator.
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CD40 Ligand (CD40LG) ELISA Kit
CD40LG Reactivity: Human Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
CD40 Ligand (CD40LG) ELISA KitCD40LG Reactivity: Mouse Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
CD40 Ligand (CD40LG) ELISA KitCD40LG Reactivity: Dog Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
CD40 Ligand (CD40LG) ELISA KitCD40LG Reactivity: Monkey Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
CD40 Ligand (CD40LG) ELISA KitCD40LG Reactivity: Chicken Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
CD40 Ligand (CD40LG) ELISA KitCD40LG Reactivity: Rabbit Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
CD40 Ligand (CD40LG) ELISA KitCD40LG Reactivity: Rat Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
CD40 Ligand (CD40LG) ELISA KitCD40LG Reactivity: Pig Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
CD40 Ligand (CD40LG) ELISA KitCD40LG Reactivity: Human AA 108-261 Colorimetric Sandwich ELISA 62.5-4000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
CD40 Ligand (CD40LG) ELISA KitCD40LG Reactivity: Human Soluble Colorimetric Sandwich ELISA 32-2000 pg/mL Cell Lysate, Plasma, Serum
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Restrictions
- For Research Use only
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Handling Advice
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1. Read the instructions carefully before beginning the assay.
2. This kit is for research use only, not for human or diagnostic use.
3. Great care has been taken to ensure the quality and reliability of this product. However, it is possible that in certain cases, unusual results may be obtained due to high levels of interfering factors. -
Storage
- 4 °C
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Storage Comment
- Kit components can be stored at 4°C until expiration date.
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Expiry Date
- The expiry date is stated on the label.
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- CD40 Ligand (CD40LG)
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Alternative Name
- TRAP
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Background
- The TRAP Staining Kit is used for the staining of tartrate-resistant acid phosphatase in osteoclasts. Bone mass is controlled by the balance between the activity of osteoblasts and the activity of osteoclasts. Alkaline phosphatase and tartrate-resistant acid phosphatase are used as markers for osteoblasts and osteoclasts, respectively. The TRAP Staining Kit supplements the Osteoclast Culture Kit.
Target
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