Total Antioxidant Capacity ELISA Kit

Catalog No. ABIN956217

This Chemical ELISA Kit is a Colorimetric ELISA Kit designed to quantify Chemical in .

Quick Overview for Total Antioxidant Capacity ELISA Kit (ABIN956217)

Target: Total Antioxidant Capacity

Reactivity: Chemical

Detection Method: Colorimetric

Method Type: Competition ELISA

Detection Range: 21.9-4378 μmol/L

Application: ELISA

Product Details

Minimum Detection Limit: 21.9 μmol/L

Analytical Method: Quantitative

Sensitivity: 21.9 μmol/L

Characteristics: Oxidative stress plays an important role in various diseases and aging. The control of oxidative stress is expected to be useful to prevent diseases and aging. Oxidative stress is caused by the imbalance between reactive oxygen species (ROS) and antioxidant defense system. For accurate assessment of oxidative stress, measurement of ROS, oxidative damage and antioxidant activity may be essential. PAO can detect not only hydrophilic antioxidants such as Vitamin C and glutathione, but can also detect hydrophobic antioxidants such as Vitamin E. Applicable for the assessment of total antioxidants of serum, foods, and beverage samples. For research use only.Samples are mixed with Cu++ Solution. Cu++ are reduced by antioxidants to form Cu+. Reduced Cu+ react with Chromatic Solution (Bathocuproine), and can be detected by absorbance at wavelength 480 to 490 nm. Antioxidant capacity can be calculated from the Cu+ formed.

Components: 1. Calibrator (Uric acid powder): 1 vial (for 2 mM), Dissolve with distilled water.
2. Sample diluent: 60 mL x 1 bottle, Ready to use.
3. Cu++ solution: 5 mL x 1 bottle, Ready to use.
4. Stop solution: 5 mL x 1 bottle, Ready to use.
5. Microtiter plate: 1 plate

Material not included: A. A microplate reader (measuring wavelength 490 nm)
B. Pipettes and pipette chips
C. Plastic test tubes
D. Distilled water
E. NaOH, HCl solution and pH meter (Not required if calibrators are prepared with distilled water only.).

Application Details

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: RT

Storage Comment: Store at room temperature until the expiration date listed on the kit box label.

Expiry Date: The expiry date is stated on the label.

Target Details

Target: Total Antioxidant Capacity

Alternative Name: Total Antioxidant Capacity (PAO)