Mumps IgG ELISA Kit

Catalog No. ABIN996995

This Colorimetric ELISA kit is designed for the quantitative measurement of Mumps Virus .

Quick Overview for Mumps IgG ELISA Kit (ABIN996995)

Target: Mumps IgG

Reactivity: Mumps Virus

Detection Method: Colorimetric

Method Type: Competition ELISA

Application: ELISA

Product Details

Purpose: Mumps IgG Enzyme linked Immunosorbent Assay (ELISA) is intended for the detection and quantitative determination of IgG antibody to Mumps virus in human sera.

Analytical Method: Quantitative

Specificity: 100%

Sensitivity: 100%

Application Details

Sample Volume: 10 μL

Assay Time: 1 - 2 h

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C

Expiry Date: 12-14 months

Target Details

Target: Mumps IgG

Target Type: Antibody

Background: The mumps virus is a member of the paramyxovirus group and the etiological agent of mumps in man. Mumps is a generalized illness usually accompanied by parotid (salivary gland) swelling and mild symptoms. It is also one of the most common causes of aseptic meningitis, encephalitis, and inflammation of the testes (orchitis), pancreas, and ovaries.
Parotitis as a presenting symptom in mumps infections is usually sufficiently diagnostic to preclude serological confirmation. However, a third of mumps infections are subclinical or unrecognized and may require viral isolation and/or some other serological procedure to confirm or rule out mumps infection. An example of this is presenting orchitis or meningoencephalitis, the two most common complications of mumps infection, without salivary gland involvement.Virus isolation is time consuming and cumbersome and is usually an impractical procedure for the typical clinical laboratory. Current methods for serodiagnosis of mumps infections are in-vitro serum neutralization, hemagglutination-inhibition (HAI), indirect immunofluorescence, and complement fixation (CF) tests. Of these methods, neutralization is reportedly the most specific. However, the neutralization test requires 4-5 days to complete the test. HAI and CF are reportedly less sensitive than the neutralization test. These methods lack specificity, which limits their usefulness in determining immune status. The HAI test also requires pretreatment of test sera to remove nonspecific hemagglutination inhibitors from some sera.

Infection with mumps virus, whether symptomatic or subclinical, is generally thought to offer lifelong immunity. As first described by Engvall and Perlman and Van Weeman, Enzyme Immunoassays can be both specific and sensitive for the detection and measurement of serum proteins. The sensitivity, specificity, and reproducibility of enzyme-linked immunoassays can be comparable to other serological tests for antibody, such as immunofluorescence, complement fixation, hemagglutination and neutralization.
ELISA is as sensitive as the neutralization test and more sensitive than CF and HAI which makes it a reliable test for determination of immune status.