Lhcb3 antibody
(2 references)Quick Overview for Lhcb3 antibody (ABIN7477847)
Target
Reactivity
Host
Clonality
Application
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Purpose
- Anti-Lhcb3 | LHCII type III chlorophyll a/b-binding protein
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Cross-Reactivity (Details)
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Arabidopsis thaliana, Arachis hypogaea, Chlorella vulgaris, Cucumis sativa, Dactylis glomeRata, Hordeum vulgare, Lycopersicon esculentum (Solanum lycopersicon), Mesembryanthemum crystallinum, Nicotiana tabacum, Oryza sativa, Pisum sativum, Phaseolus vulgaris, Physcomirella patens, Prasinoderma sp., Pyramimonas sp., Spinacia oleracea, Triticum aestivum, Triticale, Zea may, Verbascum lychnitis
Not reactive in: No confirmed exceptions from predicted reactivity are currently known -
Predicted Reactivity
- Cucumis melo,Dicots,Gymnosperms,Mosses
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Purification
- Immunogen affinity purified serum
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Immunogen
- BSA-conjugated synthetic peptide derived from a highly conserved sequence of Lhcb3 proteins from angiosperms (monocots and dicots) and gymnosperms
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Isotype
- IgG
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Application Notes
- 1 : 2000 (WB)
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- For reconstitution add 50 μL of sterile water
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Buffer
- Immunogen affinity purified serum in PBS pH 7.4
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Handling Advice
- Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube
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Storage
- -20 °C
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Storage Comment
- Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
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: "Toxic tetrapyrrole accumulation in protoporphyrinogen IX oxidase-overexpressing transgenic rice plants." in: Plant molecular biology, Vol. 67, Issue 5, pp. 535-46, (2008) (PubMed).
: "Phosphorylation-dependent regulation of excitation energy distribution between the two photosystems in higher plants." in: Biochimica et biophysica acta, Vol. 1777, Issue 5, pp. 425-32, (2008) (PubMed).
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: "Toxic tetrapyrrole accumulation in protoporphyrinogen IX oxidase-overexpressing transgenic rice plants." in: Plant molecular biology, Vol. 67, Issue 5, pp. 535-46, (2008) (PubMed).
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- Lhcb3 (Lhcii Type III Chlorophyll A/B-Binding Protein (Lhcb3))
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Background
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Background: The major light-harvesting antenna complex II (LHCII) in photsynthetic eukaryotes is located in the thylakoid membrane of the chloroplast. It is a heterotrimeric complex formed by up to 3 different individual subtypes of chlorophyll a/b-binding proteins: Lhcb1, Lhcb2, and Lhcb3. While Lhcb1 and Lhcb2 are quite similar and regularily present in multiple gene-copies, the Lhcb3 protein differs in pigment-composition and molecular size and often is coded by only a single gene. Lhcb3 seems not to be present in the mobile LHCII trimers involved in state 1-state 2 transitions. A molecular characterisation of the LHCII proteins can be found in Caffarri et al. (2004) A Look within LHCII: Differential Analysis of the Lhcb1-3 Complexes Building the Major Trimeric Antenna Complex of Higher-Plant Photosynthesis. Biochemistry 43 (29): 9467-9476.
Additional information: Antibody format is a total IgG fraction, which means that it is a pool of polyclonal antibodies obtained by purification of serum on Protein G, not on a specific antigen column.
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Molecular Weight
- 28.7 | 26 kDa for Arabidopsis thaliana
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Gene ID
- 835515
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UniProt
- Q9S7M0
Target
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