Lhcb4 antibody
The Rabbit Polyclonal anti-Lhcb4 antibody has been validated for WB. It is suitable to detect Lhcb4 in samples from Arabidopsis thaliana, Green bean, Oryza sativa, Pisum sativum and Zea mays. There is 1 publication available.
(1 reference)
Catalog No. ABIN5684016
Quick Overview for Lhcb4 antibody (ABIN5684016)
Target
Lhcb4
(Cp29 Chlorophyll A/B Binding Protein of Plant Psii (Lhcb4))
Reactivity
Arabidopsis thaliana, Green bean, Oryza sativa, Pisum sativum, Zea mays
Host
Rabbit
Clonality
Polyclonal
Application
Western Blotting (WB)
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Specificity
- The antibody is very specific for salmonid inducible form - hsp70.
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Cross-Reactivity (Details)
- No cross-reactivity with: Chlamydomonas reinhardtii
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Characteristics
- Expected / apparent Molecular Weight of the Antigene: 31.9 / 29 kDa for Arabidopsis thaliana
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Purification
- affinity purified
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Immunogen
- BSA-conjugated synthetic peptide derived from a highly conserved sequence of Lhb4 proteins from angiosperms (monocots and dicots) and gymnosperms, including Arabidopsis thaliana (Lhcb4.1 At5g01530 and Lhcb4.2 At5g08940).
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Isotype
- IgG
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Application Notes
- Recommended Dilution: 1 : 2000 - 1 : 5000 with standard ECL (WB).
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Comment
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for image of detection please refer to Kusaba et al. (2007)
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- For reconstitution add 200 µL of sterile water
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Buffer
- PBS pH 7.4
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Handling Advice
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Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles. -
Storage
- -20 °C
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: "NYC4, the rice ortholog of Arabidopsis THF1, is involved in the degradation of chlorophyll - protein complexes during leaf senescence." in: The Plant journal : for cell and molecular biology, Vol. 74, Issue 4, pp. 652-62, (2013) (PubMed).
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: "NYC4, the rice ortholog of Arabidopsis THF1, is involved in the degradation of chlorophyll - protein complexes during leaf senescence." in: The Plant journal : for cell and molecular biology, Vol. 74, Issue 4, pp. 652-62, (2013) (PubMed).
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- Lhcb4 (Cp29 Chlorophyll A/B Binding Protein of Plant Psii (Lhcb4))
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Background
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AGI Code: At5g01530
Lhcb4 (CP29) is one of the 3 minor chlrorophyll a/b-binding proteins associated with Photosystem II in plants and algae. Lhcb4 has been suggested to act in the regulation of the chl a excited state concentration of PSII because of its ability of sensing lumenal pH resulting in reversible phosphorylation. In Arabidopsis thaliana 2 genes code for two isoforms Lhcb4.1 and Lhcb4.2. A third isoform (Lhcb4.3, At5g40100), probably only present in dicots, has found to be differently regulated and therefore has been denoted as Lhcb8. -
Molecular Weight
- expected: 31.9 kDa, apparent: 29 kDa for Arabidopsis thaliana
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UniProt
- Q9XF88, Q07473
Target
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