HeLa nuclear extract (4 hr serum response)
Quick Overview for HeLa nuclear extract (4 hr serum response) (ABIN2669789)
Lysed Cells
Lysate Type
Lysate Fraction
Lysate Treatment
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Species of Lysate
- Human Cells
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Characteristics
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Origin: Human Cervix Adenocarcinoma
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Components
- 2 x 100 μg of HeLa nuclear extract (4 hr serum response) at 2.5 μg/μl
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C1orf54 (1-131) 293T Cell Transient Overexpression Lysate(Denatured)
C1orf54 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reactivity: Human
ID3 293T Cell Transient Overexpression Lysate(Denatured)ID3 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reactivity: Human
HDAC1 293T Cell Transient Overexpression Lysate(Denatured)HDAC1 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reactivity: Human
HSPD1 293T Cell Transient Overexpression Lysate(Denatured)HSPD1 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reactivity: Human
HAL 293T Cell Transient Overexpression Lysate(Denatured)HAL WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reactivity: Human
GYPA 293T Cell Transient Overexpression Lysate(Denatured)GYPA WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reactivity: Human
MEIS1 293T Cell Transient Overexpression Lysate(Denatured)MEIS1 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reactivity: Human
IMPDH1 293T Cell Transient Overexpression Lysate(Denatured)IMPDH1 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reactivity: Human
HLA-E 293T Cell Transient Overexpression Lysate(Denatured)HLA-E WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reactivity: Human
MDM4 293T Cell Transient Overexpression Lysate(Denatured)MDM4 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reactivity: Human
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Restrictions
- For Research Use only
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Concentration
- 2.5 μg/μL
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Buffer
- Nuclear extracts are supplied in dilution buffer (20 mM Hepes ( pH 7.9), 100 mM KCl, 1 mM MgCl2, 20 % glycerol, 0.5 mM PMSF and 0.5 mM DTT). Cells were cultured for 24 hours in low serum (0.5 %) conditions and then serum-stimulated (10 %) for 4 hours prior to harvesting.
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Preservative
- Dithiothreitol (DTT)
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Precaution of Use
- This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Handling Advice
- It ist recommended to aliquot the extracts into single-use fractions and then storing them at -80 °C. This eliminates repeated freeze/thaw cycles.
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Storage
- -80 °C
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Storage Comment
- To ensure stability, extracts should be stored at -80°C.
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