Nitrate Reductase antibody
The Rabbit Polyclonal anti-Nitrate Reductase antibody has been validated for WB. It is suitable to detect Nitrate Reductase in samples from Arabidopsis thaliana and Barley.
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Catalog No. ABIN334562
Quick Overview for Nitrate Reductase antibody (ABIN334562)
Target
Nitrate Reductase (NR)
(Nitrate Reductase, Assimilatory (NR))
Reactivity
Arabidopsis thaliana, Barley
Host
Rabbit
Clonality
Polyclonal
Application
Western Blotting (WB)
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Characteristics
- Expected / apparent Molecular Weight of the Antigene: 103 kDa / 117 kDa
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Purification
- serum
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Immunogen
- KLH-conjugated synthetic peptide derived from conserved domain in NADH-NR protein sequences including A.thaliana NR1 P11832, At1g77760 and NR2 P11035, At1g37130
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Application Notes
- Recommended Dilution: 1 : 1000 (WB).
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Comment
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ECL based detection systems are adviced to use since to low signal intensity can be obtaied with BCIP/NBT system.For working with diatom samples ECL Advance (GE Healthcare) or other more sensitive ECL detection reagent is recommended.In Chlamydmonas reinhardtii anti-NR antibody is also reacting with L-Aminoacid Oxidase (a nitrogen scavenging enzyme induced during nitrogen starvation).
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- For reconstitution add 100 µL of sterile water
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Handling Advice
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Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles. -
Storage
- -20 °C
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- Nitrate Reductase (NR) (Nitrate Reductase, Assimilatory (NR))
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Background
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AGI Code: At1g77760
Assimilatory nitrate reductase (NR, EC.1.6.6.1) catalyses the reduction of nitrate to nitrite in the cytoplasm. Plants contain 2 forms of NR: NADH-NR (most common form in plants and algae, predominantly found in green tissues) and NAD(P)H-NR (uses NADH or NADPH as the electron donor, constitutively expressed in plants at a low level). NADH-NR is a homodimer of two identical subunits (100-115 kDa each, hold together by a Mo-cofactor) each of them coded by up to three genes (NR1-3, NIA1-NIA3). -
Molecular Weight
- expected: 103 kDa , apparent: 117 kDa
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UniProt
- P11832, P11035
Target
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