Nitrate Reductase antibody
The Rabbit Polyclonal anti-Nitrate Reductase antibody is suitable to detect Nitrate Reductase in samples from Alfalfa, Arabidopsis thaliana, Barley, Chlamydomonas reinhardtii, Gracilaria gracilis (Red alga), Leptodictyum riparium, Mung bean, Panax notoginseng, Phaeodactylum tricornutum, Poplar (Populus), Potato, Thalassiosira pseudonana, Tomato, Trebouxia and Vitis vinifera. It has been validated for WB.
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Catalog No. ABIN334562
$646.39
Plus shipping costs $50.00
100 μg
Shipping to:
United States
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Quick Overview for Nitrate Reductase antibody (ABIN334562)
Target
Nitrate Reductase (NR)
(Nitrate Reductase, Assimilatory (NR))
Reactivity
Alfalfa, Arabidopsis thaliana, Barley, Chlamydomonas reinhardtii, Gracilaria gracilis (Red alga), Leptodictyum riparium, Mung bean, Panax notoginseng, Phaeodactylum tricornutum, Poplar (Populus), Potato, Thalassiosira pseudonana, Tomato, Trebouxia, Vitis vinifera
Host
Rabbit
Clonality
Polyclonal
Application
Western Blotting (WB)
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Purpose
- Anti-NR | Nitrate reductase, assimilatory
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Cross-Reactivity (Details)
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Arabidopsis thaliana, Chlamydomonas reinardtii, red alga Gracilaria gracilis, Hordeum vulgare,Leptodictyum riparium (Hedw.) Warnst (moss), Medicago sativa, Phaeodactylum tricornutum Bohlin accession Pt1 8.6, Panax notoginseng, Populus yunanensis Dode, Solanum lycopersicum, Solanum tuberosum, Thalassiosira sp. (diatom), Trebouxia sp.,Vigna radiata, Vitis vinigera
Not reactive in: Aspergilus niger, Emiliania huxleyi, Tisochrysis lutea -
Predicted Reactivity
- Arabis alpina,Brachypodium distachyon,Brassica napus,Brassica rapa subsp. pekinensis,Capsella rubella,Citrus clementina,Citrus sinensis,Chlorella vulgaris,Dunaliella salina,marine Diatoms,Coffea canephora,Eucalyptus grandis,Glycine max,Glycine soja,Gossypium arboretum,Helianthus annuus,Lycopersicum esculentum,Morus alba,Nannochloropsis gaditana,Nicotiana tabacum,Nicotiana attenuata,Nicotiana benthamiana,Oryza sativa,Phaseolus vulgaris,Phytophthora infestans,Physcomitrium patens,Prunus persica,Ricinus communis,Sorghum bicolor,Spinacia oleracea,Solanum lycopersicum,Symbiodinium microadriaticum,Theobroma cacao,Zea mays
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Purification
- Immunogen affinity purified serum
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Immunogen
- KLH-conjugated synthetic peptide derived from conserved domain in NADH-NR protein sequences including A.thaliana NR1 P11832
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Application Notes
- 1 : 500 -1 : 1000 (WB)
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Comment
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ECL based detection systems are adviced to use since to low signal intensity can be obtaied with BCIP/NBT system.For working with diatom samples ECL Advance (GE Healthcare) or other more sensitive ECL detection reagent is recommended.In Chlamydmonas reinhardtii anti-NR antibody is also reacting with L-Aminoacid Oxidase (a nitrogen scavenging enzyme induced during nitrogen starvation).
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- For reconstitution add 50 μL of sterile water
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Buffer
- Immunogen affinity purified serum in PBS pH 7.4.
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Handling Advice
- Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube
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Storage
- -20 °C
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Storage Comment
- Store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
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- Nitrate Reductase (NR) (Nitrate Reductase, Assimilatory (NR))
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Background
- Assimilatory nitrate reductase (NR), (EC.1.6.6.1) catalyses the reduction of nitrate to nitrite in the cytoplasm. Plants contain 2 forms of NR: NADH-NR (most common form in plants and algae, predominantly found in green tissues) and NAD(P)H-NR (uses NADH or NADPH as the electron donor, constitutively expressed in plants at a low level). NADH-NR is a homodimer of two identical subunits (100-115 kDa each, hold together by a Mo-cofactor) each of them coded by up to three genes (NR1-3, NIA1-NIA3).
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Molecular Weight
- 103 kDa | 117 kDa
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Gene ID
- 844112
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UniProt
- P11832, P11035
Target
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