Human SP-D EZ Set™ ELISA Kit (DIY Antibody Pairs)
Quick Overview for Human SP-D EZ Set™ ELISA Kit (DIY Antibody Pairs) (ABIN5510794)
Target
Binding Specificity
Reactivity
Host
Application
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Detection Method
- Colorimetric
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Method Type
- Sandwich ELISA
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Purpose
- EZ Set™ ELISA kit containing the core components for developing solid phase sandwich ELISA assay to quantitatively detects SP-D in Human samples.
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Brand
- EZ-Set™
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Specificity
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Expression system for standard: NSO
Immunogen sequence: A21-F375 -
Cross-Reactivity (Details)
- There is no detectable cross-reactivity with other relevant proteins.
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Characteristics
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Sequence similarities: Belongs to the SFTPD family.
Tissue Specificity: Expressed in lung, brain, pancreas and adipose tissue (mainly mature adipocytes). .
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Components
- Capture Antibody, Biotinylated Detection Antibody, Lyophilized Standard, Avidin-Biotin-Peroxidase Complex (ABC)
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Material not included
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1. Microplate reader in standard size.
2. Automated plate washer.
3. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
4. Clean tubes and Eppendorf tubes.
5. Washing buffer (neutral PBS or TBS).
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SFTPD (Human) Matched Antibody Pair
ELISA Reactivity: Human ELISA unconjugated Host: Mouse
SFTPD (Human) Matched Antibody PairELISA Reactivity: Human ELISA unconjugated Host: Mouse
SFTPD (Human) Matched Antibody PairELISA Reactivity: Human ELISA unconjugated Host: Mouse
SFTPD (Human) Matched Antibody PairELISA Reactivity: Human ELISA unconjugated Host: Mouse
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Comment
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The EZ Set™ ELISA antibody pair set contains the necessary core reagents to develop and run an ELISA, including ELISA-optimized matched antibody pairs, recombinant protein standards, and HRP conjugated Avidin-Biotin-Complex (ABC).
The remaining necessary components could be obtained via the EZ Set™ Accessory Kit (ABIN5510802)
The EZ Set™ Accessory Kit provides:
Antibody diluent buffer
ABC diluent buffer
Sample diluent buffer
TMB
TMB stop solution
5x 96 well plates, plate sealers -
Restrictions
- For Research Use only
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Buffer
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Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
Preparation of 0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L. -
Storage
- 4 °C/-20 °C
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Storage Comment
- Store at 4° for 6 months, at -20° for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
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Expiry Date
- 12 months
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- SFTPD (Surfactant Protein D (SFTPD))
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Alternative Name
- SFTPD
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Background
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Protein Function: Contributes to the lung's defense against inhaled microorganisms, organic antigens and toxins. Interacts with compounds such as bacterial lipopolysaccharides, oligosaccharides and fatty acids and modulates leukocyte action in immune response. May participate in the extracellular reorganization or turnover of pulmonary surfactant. Binds strongly maltose residues and to a lesser extent other alpha-glucosyl moieties. .
Background: Surfactant, pulmonary-associated protein D, also known as SFTPD or SP-D, is a protein which in humans is encoded by the SFTPD gene. It is mapped to 10q22.2-q23.1. The protein encoded by this gene is part of the innate immune response, protecting the lungs against inhaled microorganisms and chemicals. The encoded protein may also be involved in surfactant metabolism.
Synonyms: Pulmonary surfactant-associated protein D,PSP-D,SP-D,Collectin-7,Lung surfactant protein D,SFTPD,COLEC7, PSPD, SFTP4,
Full Gene Name: Pulmonary surfactant-associated protein D
Cellular Localisation: Secreted, extracellular space, extracellular matrix. Secreted, extracellular space, surface film. -
UniProt
- P35247
Target
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