Mouse Prolactin EZ Set™ ELISA Kit (DIY Antibody Pairs)
Quick Overview for Mouse Prolactin EZ Set™ ELISA Kit (DIY Antibody Pairs) (ABIN5510772)
Target
Binding Specificity
Reactivity
Host
Application
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Detection Method
- Colorimetric
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Method Type
- Sandwich ELISA
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Purpose
- EZ Set™ ELISA kit containing the core components for developing solid phase sandwich ELISA assay to quantitatively detects Prolactin in Mouse samples.
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Brand
- EZ-Set™
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Specificity
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Expression system for standard: E.coli
Immunogen sequence: L32-C228 -
Cross-Reactivity (Details)
- There is no detectable cross-reactivity with other relevant proteins.
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Components
- Capture Antibody, Biotinylated Detection Antibody, Lyophilized Standard, Avidin-Biotin-Peroxidase Complex (ABC)
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Material not included
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1. Microplate reader in standard size.
2. Automated plate washer.
3. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
4. Clean tubes and Eppendorf tubes.
5. Washing buffer (neutral PBS or TBS).
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PRL (Human) Matched Antibody Pair
ELISA Reactivity: Human ELISA unconjugated Host: Mouse
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Comment
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The EZ Set™ ELISA antibody pair set contains the necessary core reagents to develop and run an ELISA, including ELISA-optimized matched antibody pairs, recombinant protein standards, and HRP conjugated Avidin-Biotin-Complex (ABC).
The remaining necessary components could be obtained via the EZ Set™ Accessory Kit (ABIN5510802)
The EZ Set™ Accessory Kit provides:
Antibody diluent buffer
ABC diluent buffer
Sample diluent buffer
TMB
TMB stop solution
5x 96 well plates, plate sealers -
Restrictions
- For Research Use only
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Buffer
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Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
Preparation of 0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L. -
Storage
- 4 °C/-20 °C
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Storage Comment
- Store at 4° for 6 months, at -20° for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
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Expiry Date
- 12 months
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- Prolactin (PRL)
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Alternative Name
- Prl
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Background
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Protein Function: Prolactin acts primarily on the mammary gland by promoting lactation.
Background: Prolactin, also calle PRL, is a protein that in humans is encodes by the PRL gene. This gene is mapped to 6p22.3. This gene encodes the anterior pituitary hormone prolactin. It may play a role in cell survival by suppressing apoptosis, and it is essential for lactation. Prolactin also acts in a cytokine-like manner and as an important regulator of the immune system. Prolactin has important cell cycle related functions as a growth-, differentiating- and anti-apoptotic factor. As a growth factor binding to cytokine like receptors it has also profound influence on hematopoiesis, angiogenesis and is involved in the regulation of blood clotting through several pathways.
Synonyms: Prolactin,PRL,Prl,
Full Gene Name: Prolactin
Cellular Localisation: Secreted. -
UniProt
- P06879
Target
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