Mouse CXCL4/PF4 EZ Set™ ELISA Kit (DIY Antibody Pairs)
Quick Overview for Mouse CXCL4/PF4 EZ Set™ ELISA Kit (DIY Antibody Pairs) (ABIN5510776)
Target
Binding Specificity
Reactivity
Host
Application
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Detection Method
- Colorimetric
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Method Type
- Sandwich ELISA
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Purpose
- EZ Set™ ELISA kit containing the core components for developing solid phase sandwich ELISA assay to quantitatively detects CXCL4/PF4 in Mouse samples.
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Brand
- EZ-Set™
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Specificity
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Expression system for standard: E.coli
Immunogen sequence: V30-S105 -
Cross-Reactivity (Details)
- There is no detectable cross-reactivity with other relevant proteins.
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Components
- Capture Antibody, Biotinylated Detection Antibody, Lyophilized Standard, Avidin-Biotin-Peroxidase Complex (ABC)
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Material not included
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1. Microplate reader in standard size.
2. Automated plate washer.
3. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
4. Clean tubes and Eppendorf tubes.
5. Washing buffer (neutral PBS or TBS).
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PF4 (Human) IP-WB Antibody Pair
IP Reactivity: Human IP unconjugated Host: Rabbit, Mouse
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Comment
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The EZ Set™ ELISA antibody pair set contains the necessary core reagents to develop and run an ELISA, including ELISA-optimized matched antibody pairs, recombinant protein standards, and HRP conjugated Avidin-Biotin-Complex (ABC).
The remaining necessary components could be obtained via the EZ Set™ Accessory Kit (ABIN5510802)
The EZ Set™ Accessory Kit provides:
Antibody diluent buffer
ABC diluent buffer
Sample diluent buffer
TMB
TMB stop solution
5x 96 well plates, plate sealers -
Restrictions
- For Research Use only
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Buffer
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Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
Preparation of 0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L. -
Storage
- 4 °C/-20 °C
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Storage Comment
- Store at 4° for 6 months, at -20° for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
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Expiry Date
- 12 months
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- PF4 (Platelet Factor 4 (PF4))
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Alternative Name
- Pf4
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Background
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Protein Function: Released during platelet aggregation. Neutralizes the anticoagulant effect of heparin because it binds more strongly to heparin than to the chondroitin-4-sulfate chains of the carrier molecule. Chemotactic for neutrophils and monocytes. Inhibits endothelial cell proliferation (By similarity). .
Background: Platelet factor 4(PF4) is a small cytokine belonging to the CXC chemokine family that is also known as chemokine(C-X-C motif) ligand 4(CXCL4). The PF4 gene was localized on map to 4q12-q13. Chemokines play fundamental roles in the development, homeostasis, and function of the immune system, and they have effects on cells of the central nervous system as well as on endothelial cells involved in angiogenesis or angiostasis. Platelet factor-4 is a 70-amino acid protein that is released from the alpha-granules of activated platelets and binds with high affinity to heparin. Its major physiologic role appears to be neutralization of heparin-like molecules on the endothelial surface of blood vessels, thereby inhibiting local antithrombin III activity and promoting coagulation. As a strong chemoattractant for neutrophils and fibroblasts, PF4 probably has a role in inflammation and wound repair.
Synonyms: Platelet factor 4,PF-4,C-X-C motif chemokine 4,Pf4,Cxcl4, Scyb4,
Full Gene Name: Platelet factor 4
Cellular Localisation: Secreted. -
UniProt
- Q9Z126
Target
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