Human TAFI/CPB2 EZ Set™ ELISA Kit (DIY Antibody Pairs)
Quick Overview for Human TAFI/CPB2 EZ Set™ ELISA Kit (DIY Antibody Pairs) (ABIN5510800)
Target
Binding Specificity
Reactivity
Host
Application
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Detection Method
- Colorimetric
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Method Type
- Sandwich ELISA
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Purpose
- EZ Set™ ELISA kit containing the core components for developing solid phase sandwich ELISA assay to quantitatively detects TAFI/CPB2 in Human samples.
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Brand
- EZ-Set™
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Specificity
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Expression system for standard: NSO
Immunogen sequence: M1-V423 -
Cross-Reactivity (Details)
- There is no detectable cross-reactivity with other relevant proteins.
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Characteristics
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Tissue Specificity: Plasma, synthesized in the liver.
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Components
- Capture Antibody, Biotinylated Detection Antibody, Lyophilized Standard, Avidin-Biotin-Peroxidase Complex (ABC)
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Material not included
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1. Microplate reader in standard size.
2. Automated plate washer.
3. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
4. Clean tubes and Eppendorf tubes.
5. Washing buffer (neutral PBS or TBS).
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CPB2 (Human) IP-WB Antibody Pair
IP Reactivity: Human IP unconjugated Host: Rabbit, Mouse
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Comment
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The EZ Set™ ELISA antibody pair set contains the necessary core reagents to develop and run an ELISA, including ELISA-optimized matched antibody pairs, recombinant protein standards, and HRP conjugated Avidin-Biotin-Complex (ABC).
The remaining necessary components could be obtained via the EZ Set™ Accessory Kit (ABIN5510802)
The EZ Set™ Accessory Kit provides:
Antibody diluent buffer
ABC diluent buffer
Sample diluent buffer
TMB
TMB stop solution
5x 96 well plates, plate sealers -
Restrictions
- For Research Use only
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Buffer
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Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
Preparation of 0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L. -
Storage
- 4 °C/-20 °C
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Storage Comment
- Store at 4° for 6 months, at -20° for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
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Expiry Date
- 12 months
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- CPB2 (Carboxypeptidase B2 (Plasma) (CPB2))
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Alternative Name
- CPB2
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Background
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Protein Function: Cleaves C-terminal arginine or lysine residues from biologically active peptides such as kinins or anaphylatoxins in the circulation thereby regulating their activities. Down- regulates fibrinolysis by removing C-terminal lysine residues from fibrin that has already been partially degraded by plasmin. .
Background: Carboxypeptidase B2 (CPB2), also known as carboxypeptidase U (CPU), plasma carboxypeptidase B (pCPB) or thrombin-activatable fibrinolysis inhibitor (TAFI), is an enzyme that, in humans, is encoded by the gene CPB2. CPB2 is synthesized by the liver and circulates in the plasma as a plasminogen-bound zymogen. When it is activated by proteolysis at residue Arg92 by the thrombin/thrombomodulin complex, CPB2 exhibits carboxypeptidase activity. Activated CPB2 reduces fibrinolysis by removing the fibrin C-terminal residues that are important for the binding and activation of plasminogen.
Synonyms: Carboxypeptidase B2,3.4.17.20 ,Carboxypeptidase U,CPU,Plasma carboxypeptidase B,pCPB,Thrombin-activable fibrinolysis inhibitor,TAFI,CPB2,
Full Gene Name: Carboxypeptidase B2
Cellular Localisation: Secreted. -
UniProt
- Q96IY4
Target
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