PCDHG antibody (C-Term, Cytoplasmic Domain, pan)
The Mouse Monoclonal anti-PCDHG antibody has been validated for ICC, IF and WB. It is suitable to detect PCDHG in samples from Mouse and Rat.
Catalog No. ABIN5539938
Quick Overview for PCDHG antibody (C-Term, Cytoplasmic Domain, pan) (ABIN5539938)
Target
PCDHG
(Protocadherin gamma Cluster (PCDHG))
Reactivity
Mouse, Rat
Host
Mouse
Clonality
Monoclonal
Application
Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)
Clone
S159-5
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Binding Specificity
- AA 808-931, C-Term, Cytoplasmic Domain, pan
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Specificity
- This antibody detects a ~100 kDa protein. Cross-reacts with all Gamma- protocadherin-A, -B and -C proteins. Species: Mouse and Rat.
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Purification
- Protein G Chromatography
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Immunogen
- Fusion protein corresponding to amino acids 808-931 (C-terminal cytoplasmic constant domain) of mouse Protocadherin-gamma-A1 that is shared by all 22 Gamma-protocadherins (A-subfamily: amino acids ~807-930, B-subfamily: amino acids ~789-912 and C subfamily).
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Isotype
- IgG1
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Application Notes
- Western blot: 1/1000, 1 μg/mL was sufficient for detection of PCDHG (pan) in 20 μg of Rat brain lysate by colorimetric immunoblot analysis using HRP conjugated secondary antibody. Immunocytochemistry .
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Restrictions
- For Research Use only
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Format
- Liquid
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Buffer
- PBS pH 7.4, 50 % Glycerol, 0.09 % Sodium Azide
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Preservative
- Sodium azide
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Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Storage
- -20 °C
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Storage Comment
- Upon receipt, store undiluted (in aliquots) at -20°C. Avoid repeated freezing and thawing. Shelf life: One year from despatch.
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- PCDHG (Protocadherin gamma Cluster (PCDHG))
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Alternative Name
- pcdhg
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Background
- The protocadherin gamma gene cluster is one of three related clusters tandemly linked on chromosome five. These gene clusters have an immunoglobulin-like organization, suggesting that a novel mechanism may be involved in their regulation and expression. The gamma gene cluster includes 22 genes divided into 3 subfamilies. Subfamily A contains 12 genes, subfamily B contains 7 genes and 2 pseudogenes, and the more distantly related subfamily C contains 3 genes. The tandem array of 22 large, variable region exons are followed by a constant region, containing 3 exons shared by all genes in the cluster. Each variable region exon encodes the extracellular region, which includes 6 cadherin ectodomains and a transmembrane region. The constant region exons encode the common cytoplasmic region. These neural cadherin-like cell adhesion proteins most likely play a critical role in the establishment and function of specific cell-cell connections in the brain. Alternative splicing has been described for the gamma cluster genes.
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Gene ID
- 93709
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UniProt
- Q91XZ0
Target
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