Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (C-Term) Peptide
GAPDH
Reactivity: Human, Mouse
Host: Synthetic
BP, WB
Catalog No. ABIN1540981
Quick Overview for Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (C-Term) Peptide (ABIN1540981)
Target
GAPDH
(Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))
Origin
Human, Mouse
Source
Synthetic
Application
Blocking Peptide (BP), Western Blotting (WB)
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Protein Region
- C-Term
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Sequence
- FNSNSHSSTF DAGAGIALND NFVKLISWYD NEYGYSNRVV DLMAYMASKE
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Characteristics
- This is a synthetic peptide designed for use in combination with anti-Gapdh Antibody. It may block above mentioned antibody from binding to its target protein in western blot and/or immunohistochecmistry under proper experimental settings. There is no guarantee for its use in other applications.
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Purification
- Purified
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Application Notes
- Each Investigator should determine their own optimal working dilution for specific applications.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Add 100 μL of sterile PBS. Final peptide concentration is 1 mg/mL in PBS.
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Concentration
- 1 mg/mL
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Buffer
- Final peptide concentration is 1 mg/mL in PBS.
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Handling Advice
- Avoid repeated freeze-thaw cycles.
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Storage
- -20 °C
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Storage Comment
- For longer periods of storage, store at -20°C. Avoid repeat freeze-thaw cycles.
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- GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH))
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Background
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Gapdh has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Gapdh also participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC.
Alias Symbols: Gapd, MGC102544, MGC102546, MGC103190, MGC103191, MGC105239
Protein Size: 333 -
Molecular Weight
- 36 kDa
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Gene ID
- 14433
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NCBI Accession
- NM_008084, NP_032110
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UniProt
- P16858
Target
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