V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) (Middle Region) Peptide
RAF1
Reactivity: Human
Host: Synthetic
BP, WB
Catalog No. ABIN2185168
Quick Overview for V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1) (Middle Region) Peptide (ABIN2185168)
Target
RAF1
(V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1))
Origin
Human
Source
Synthetic
Application
Blocking Peptide (BP), Western Blotting (WB)
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Protein Region
- Middle Region
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Sequence
- SGTQEKNKIR PRGQRDSSYY WEIEASEVML STRIGSGSFG TVYKGKWHGD
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Characteristics
- This is a synthetic peptide designed for use in combination with anti-RAF1 Antibody. It may block above mentioned antibody from binding to its target protein in western blot and/or immunohistochecmistry under proper experimental settings. There is no guarantee for its use in other applications.
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Purification
- Purified
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Add 100 μL of sterile PBS. Final peptide concentration is 1 mg/mL in PBS.
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Concentration
- 1 mg/mL
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Buffer
- Final peptide concentration is 1 mg/mL in PBS.
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Handling Advice
- Avoid repeat freeze-thaw cycles.
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Storage
- -20 °C
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Storage Comment
- For longer periods of storage, store at -20°C. Avoid repeat freeze-thaw cycles.
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- RAF1 (V-Raf-1 Murine Leukemia Viral Oncogene Homolog 1 (RAF1))
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Background
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This gene is the cellular homolog of viral raf gene (v-raf). The encoded protein is a MAP kinase kinase kinase (MAP3K), which functions downstream of the Ras family of membrane associated GTPases to which it binds directly. Once activated, the cellular RAF1 protein can phosphorylate to activate the dual specificity protein kinases MEK1 and MEK2, which in turn phosphorylate to activate the serine/threonine specific protein kinases, ERK1 and ERK2. Activated ERKs are pleiotropic effectors of cell physiology and play an important role in the control of gene expression involved in the cell division cycle, apoptosis, cell differentiation and cell migration. Mutations in this gene are associated with Noonan syndrome 5 and LEOPARD syndrome 2.
Protein Size: 567 -
Molecular Weight
- 62 kDa
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Gene ID
- 5894
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NCBI Accession
- XP_005265415
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UniProt
- B4E0X2
Target
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