Protein Kinase D1 (PRKD1) (AA 383-395), (Internal Region) Peptide
PRKD1
Reactivity: Human
Host: Synthetic
BP, Imm
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- Target See all PKC mu (PRKD1) products
- PKC mu (PRKD1) (Protein Kinase D1 (PRKD1))
- Protein Region
- AA 383-395, Internal Region
- Origin
- Human
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Source
- Synthetic
- Application
- Blocking Peptide (BP), Immunogen (Imm)
- Sequence
- QDPDPDHEDA NRT
- Predicted Reactivity
- Human
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- Application Notes
- When peptides are reconstituted in 200 μL water, the concentration would be also 0.5 mg/mL. To start, the best ratio would be 1:1 (which means molar excess of peptides relative to antibodies when identical volumes are mixed). Please mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. Best is to have two identical blots, to be incubated with equal amount of antibodies, but one with the antibodies pre-adsorbed to the peptide for 20 min. Then incubate and develop in parallel the two blots.In transfected HEK293 transiently expressing Human PKD1 (DYKDDDDK tagged), a band of approx. 100 kDa is observed. The same band is observed using anti- DYKDDDDK antibody and no bands are observed in cells transiently expressing Human PKD2 or PKD3. Recommen
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Reconstitute pellet in distilled water where required. For Blocking studies, reconstitute pellet in 200µl distilled water to obtain a 0.5mg/ml peptide solution.
- Buffer
- 100µg of Lyophilised peptide, formulation De-ionized (DI) water.
- Storage
- -20 °C
- Storage Comment
- Shipped at ambient temperature, store at -20°C.
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- Target
- PKC mu (PRKD1) (Protein Kinase D1 (PRKD1))
- Gene ID
- 5587
- NCBI Accession
- NP_002733
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