Malate Dehydrogenase 2, NAD (Mitochondrial) (MDH2) (Internal Region) Peptide
MDH2
Reactivity: Human, Mouse, Pig, Rat
Host: Synthetic
BP, Imm
Catalog No. ABIN5539453
Quick Overview for Malate Dehydrogenase 2, NAD (Mitochondrial) (MDH2) (Internal Region) Peptide (ABIN5539453)
Target
MDH2
(Malate Dehydrogenase 2, NAD (Mitochondrial) (MDH2))
Origin
Human, Mouse, Pig, Rat
Source
Synthetic
Application
Blocking Peptide (BP), Immunogen (Imm)
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Protein Region
- Internal Region
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Sequence
- TRDDLFNTNA T
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Predicted Reactivity
- Human, Mouse, Rat, Pig, Cow
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Application Notes
- When peptides are reconstituted in 200 μL water, the concentration would be also 0.5 mg/mL. To start, the best ratio would be 1:1 (which means molar excess of peptides relative to antibodies when identical volumes are mixed). Please mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. Best is to have two identical blots, to be incubated with equal amount of antibodies, but one with the antibodies pre-adsorbed to the peptide for 20 min. Then incubate and develop in parallel the two blots.Approx 35 kDa band observed in lysates of cell lines HeLa, HepG2 and NIH3T3 (calculated MW of 35.5 kDa according to Human NP_005909.2 and 35.6 kDa according to Mouse NP_032643.2 ). Recommended concentration: 0.1-0.3 μg/mL. Approx 35 kDa band observed in Human,
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Reconstitute pellet in distilled water where required. For Blocking studies, reconstitute pellet in 200µl distilled water to obtain a 0.5mg/ml peptide solution.
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Buffer
- 100µg of Lyophilised peptide, formulation De-ionized (DI) water.
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Storage
- -20 °C
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Storage Comment
- Shipped at ambient temperature, store at -20°C.
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- MDH2 (Malate Dehydrogenase 2, NAD (Mitochondrial) (MDH2))
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Gene ID
- 4191, 17448, 81829
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NCBI Accession
- NP_005909
Target
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