Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5) (Middle Region) Peptide
MAP3K5
Reactivity: Human
Host: Synthetic
BP, WB
Catalog No. ABIN5672376
Quick Overview for Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5) (Middle Region) Peptide (ABIN5672376)
Target
ASK1 (MAP3K5)
(Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5))
Origin
Human
Source
Synthetic
Application
Blocking Peptide (BP), Western Blotting (WB)
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Protein Region
- Middle Region
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Sequence
- IRTLFLGIPD ENFEDHSAPP SPEEKDSGFF MLRKDSERRA TLHRILTEDQ
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Characteristics
- This is a synthetic peptide designed for use in combination with anti- MAP3K5 Antibody. It may block above mentioned antibody from binding to its target protein in western blot and/or immunohistochecmistry under proper experimental settings. There is no guarantee for its use in other applications.
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Add 100 μL of sterile PBS. Final peptide concentration is 1 mg/mL in PBS.
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Storage
- -20 °C
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Storage Comment
- For longer periods of storage, store at -20°C. Avoid repeat freeze-thaw cycles.
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- ASK1 (MAP3K5) (Mitogen-Activated Protein Kinase Kinase Kinase 5 (MAP3K5))
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Background
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Mitogen-activated protein kinase (MAPK) signaling cascades include MAPK or extracellular signal-regulated kinase (ERK), MAPK kinase (MKK or MEK), and MAPK kinase kinase (MAPKKK or MEKK). MAPKK kinase/MEKK phosphorylates and activates its downstream protein kinase, MAPK kinase/MEK, which in turn activates MAPK. The kinases of these signaling cascades are highly conserved, and homologs exist in yeast, Drosophila, and mammalian cells. MAPKKK5 contains 1,374 amino acids with all 11 kinase subdomains. Northern blot analysis shows that MAPKKK5 transcript is abundantly expressed in human heart and pancreas. The MAPKKK5 protein phosphorylates and activates MKK4 (aliases SERK1, MAPKK4) in vitro, and activates c-Jun N-terminal kinase (JNK)/stress-activated protein kinase (SAPK) during transient expression in COS and 293 cells, MAPKKK5 does not activate MAPK/ERK.
Alias Symbols: ASK1, MEKK5, MAPKKK5
Protein Size: 621 -
Gene ID
- 4217
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NCBI Accession
- NM_005923, NP_005914
Target
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