Flap Structure-Specific Endonuclease 1 (FEN1) (C-Term) Peptide
FEN1
Reactivity: Human
Host: Synthetic
BP, WB
Catalog No. ABIN5672537
Quick Overview for Flap Structure-Specific Endonuclease 1 (FEN1) (C-Term) Peptide (ABIN5672537)
Target
FEN1
(Flap Structure-Specific Endonuclease 1 (FEN1))
Origin
Human
Source
Synthetic
Application
Blocking Peptide (BP), Western Blotting (WB)
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Protein Region
- C-Term
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Sequence
- SRQGSTQGRL DDFFKVTGSL SSAKRKEPEP KGSTKKKAKT GAAGKFKRGK
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Characteristics
- This is a synthetic peptide designed for use in combination with anti- FEN1 Antibody. It may block above mentioned antibody from binding to its target protein in western blot and/or immunohistochecmistry under proper experimental settings. There is no guarantee for its use in other applications.
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Add 100 μL of sterile PBS. Final peptide concentration is 1 mg/mL in PBS.
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Storage
- -20 °C
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Storage Comment
- For longer periods of storage, store at -20°C. Avoid repeat freeze-thaw cycles.
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- FEN1 (Flap Structure-Specific Endonuclease 1 (FEN1))
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Background
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The protein encoded by this gene removes 5' overhanging flaps in DNA repair and processes the 5' ends of Okazaki fragments in lagging strand DNA synthesis. Direct physical interaction between this protein and AP endonuclease 1 during long-patch base excision repair provides coordinated loading of the proteins onto the substrate, thus passing the substrate from one enzyme to another. The protein is a member of the XPG/RAD2 endonuclease family and is one of ten proteins essential for cell-free DNA replication. DNA secondary structure can inhibit flap processing at certain trinucleotide repeats in a length-dependent manner by concealing the 5' end of the flap that is necessary for both binding and cleavage by the protein encoded by this gene. Therefore, secondary structure can deter the protective function of this protein, leading to site-specific trinucleotide expansions.
Alias Symbols: MF1, RAD2, FEN-1
Protein Size: 316 -
Gene ID
- 2237
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NCBI Accession
- NM_004111, NP_004102
Target
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