Proliferating Cell Nuclear Antigen (PCNA) (AA 111-122), (Internal Region) Peptide
PCNA
Reactivity: Human, Mouse, Rat
Host: Synthetic
BP, BI
Catalog No. ABIN5685392
Quick Overview for Proliferating Cell Nuclear Antigen (PCNA) (AA 111-122), (Internal Region) Peptide (ABIN5685392)
Target
PCNA
(Proliferating Cell Nuclear Antigen (PCNA))
Origin
Human, Mouse, Rat
Source
Synthetic
Application
Blocking Peptide (BP), BioImaging (BI)
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Protein Region
- AA 111-122, Internal Region
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Characteristics
- PCNA peptide corresponds to a 12 amino acid peptide near the internal region of PCNA (aa111-122).
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Application Notes
- PCNA Antibody ships at 0.5 mg/mL and the PCNA Peptide ships as a 100 μ,g pellet. When peptide is reconstituted in 200 μ,L water, the concentration will be 0.5 mg/mL, identical to that of the antibody. The best starting ratio would be 1:1 (which means molar excess of peptide relative to antibody when identical volumes are mixed). Mix equal volume of peptide and antibody at the required dilution and leave at ambient temperature for 20 minutes. Then incubate and develop in parallel two blots: one with the antibody and one with the antibody which has been pre-absorbed with the provided peptide.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- PCNA Peptide is supplied as 100 µg of dried peptide. When the peptide is reconstituted in 200 µL water, the concentration will be 0.5 mg/mL.
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Storage
- -20 °C
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Storage Comment
- Store at -20°C. When reconstituted minimize freezing and thawing.
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