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E3 Ubiquitin-Protein Ligase SIAH1 (SIAH1) (N-Term) Peptide

SIAH1 Reactivity: Human, Rat Host: Synthetic BP, BI
Catalog No. ABIN5686208
  • Target See all SIAH1 products
    SIAH1 (E3 Ubiquitin-Protein Ligase SIAH1 (SIAH1))
    Protein Region
    N-Term
    Origin
    Human, Rat
    Source
    • 5
    Synthetic
    Application
    Blocking Peptide (BP), BioImaging (BI)
    Characteristics
    SIAH1 peptide corresponds to 15 amino acids near the N-terminus region of SIAH1.
  • Application Notes
    SIAH1 Peptide is used for blocking the activity of SIAH1 antibody . The SIAH1 antibody ships at 0.5 mg/mL and the SIAH1 Peptide ships as a 100 μg pellet. When peptide is reconstituted in 200 μLwater, the concentration will be 0.5 mg/mL, identical to that of the antibody. The best starting ratio would be 1:1 (which means molar excess of peptide relative to antibody when identical volumes are mixed). Mix equal volume of peptide and antibody at the required dilution and leave at ambient temperature for 20 minutes. Then incubate and develop in parallel two blots: one with the antibody and one with the antibody which has been pre-absorbed with the provided peptide.
    Restrictions
    For Research Use only
  • Format
    Lyophilized
    Reconstitution
    SIAH1 peptide is supplied as 100 µg of dried peptide. When the peptide is reconstituted in 200 µL water, the concentration will be 0.5 mg/mL.
    Storage
    -20 °C
    Storage Comment
    Store at -20°C. When reconstituted minimize freezing and thawing.
  • Target
    SIAH1 (E3 Ubiquitin-Protein Ligase SIAH1 (SIAH1))
    Gene ID
    6477
    NCBI Accession
    NP_003022, NP_001006611
    OMIM
    63148618
    UniProt
    Q8IUQ4
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