BCL2-Like 11 (Apoptosis Facilitator) (BCL2L11) Peptide
BCL2L11
Reactivity: Human
Host: Synthetic
BP, BI
Catalog No. ABIN5686527
Quick Overview for BCL2-Like 11 (Apoptosis Facilitator) (BCL2L11) Peptide (ABIN5686527)
Target
BIM (BCL2L11)
(BCL2-Like 11 (Apoptosis Facilitator) (BCL2L11))
Origin
Human
Source
Synthetic
Application
Blocking Peptide (BP), BioImaging (BI)
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Characteristics
- BIM peptide corresponds to 11 amino acids of BIM.
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Application Notes
- BIM Peptide is used for blocking the activity of BIM antibody . The BIM antibody ships at 0.5 mg/mL and the BIM Peptide ships as a 100 μg pellet. When peptide is reconstituted in 200 μLwater, the concentration will be 0.5 mg/mL, identical to that of the antibody. The best starting ratio would be 1:1 (which means molar excess of peptide relative to antibody when identical volumes are mixed). Mix equal volume of peptide and antibody at the required dilution and leave at ambient temperature for 20 minutes. Then incubate and develop in parallel two blots: one with the antibody and one with the antibody which has been pre-absorbed with the provided peptide.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- BIM peptide is supplied as 100 µg of dried peptide. When the peptide is reconstituted in 200 µL water, the concentration will be 0.5 mg/mL.
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Storage
- -20 °C
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Storage Comment
- Store at -20°C. When reconstituted minimize freezing and thawing.
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