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Choline O-Acetyltransferase (CHAT) (C-Term) Peptide

CHAT Reactivity: Mouse, Rat Host: Synthetic BP, BI
Catalog No. ABIN5686738

Quick Overview for Choline O-Acetyltransferase (CHAT) (C-Term) Peptide (ABIN5686738)

Target

Choline Acetyltransferase (CHAT) (Choline O-Acetyltransferase (CHAT))

Origin

Mouse, Rat

Source

  • 4
Synthetic

Application

Blocking Peptide (BP), BioImaging (BI)
  • Protein Region

    C-Term

    Characteristics

    ChAT peptide corresponds to 14 amino acids near the C-terminus region of ChAT.
  • Application Notes

    ChAT Peptide is used for blocking the activity of ChAT antibody . The ChAT antibody ships at 0.5 mg/mL and the ChAT Peptide ships as a 100 μg pellet. When peptide is reconstituted in 200 μLwater, the concentration will be 0.5 mg/mL, identical to that of the antibody. The best starting ratio would be 1:1 (which means molar excess of peptide relative to antibody when identical volumes are mixed). Mix equal volume of peptide and antibody at the required dilution and leave at ambient temperature for 20 minutes. Then incubate and develop in parallel two blots: one with the antibody and one with the antibody which has been pre-absorbed with the provided peptide.

    Restrictions

    For Research Use only
  • Format

    Lyophilized

    Reconstitution

    ChAT peptide is supplied as 100 µg of dried peptide. When the peptide is reconstituted in 200 µL water, the concentration will be 0.5 mg/mL.

    Storage

    -20 °C

    Storage Comment

    Store at -20°C. When reconstituted minimize freezing and thawing.
  • Target

    Choline Acetyltransferase (CHAT) (Choline O-Acetyltransferase (CHAT))

    Gene ID

    12647

    NCBI Accession

    NP_034021

    OMIM

    45476575

    UniProt

    Q03059
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