Secreted phosphoprotein 1 (SPP1) (C-Term) Peptide
SPP1
Reactivity: Human
Host: Synthetic
BP, BI
Catalog No. ABIN5686959
Quick Overview for Secreted phosphoprotein 1 (SPP1) (C-Term) Peptide (ABIN5686959)
Target
Osteopontin (SPP1)
(Secreted phosphoprotein 1 (SPP1))
Origin
Human
Source
Synthetic
Application
Blocking Peptide (BP), BioImaging (BI)
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Protein Region
- C-Term
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Characteristics
- Osteopontin peptide corresponds to 12 amino acids near the C-terminus region of Osteopontin.
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Application Notes
- Osteopontin Peptide is used for blocking the activity of Osteopontin antibody . The Osteopontin antibody ships at 0.5 mg/mL and the Osteopontin Peptide ships as a 100 μg pellet. When peptide is reconstituted in 200 μLwater, the concentration will be 0.5 mg/mL, identical to that of the antibody. The best starting ratio would be 1:1 (which means molar excess of peptide relative to antibody when identical volumes are mixed). Mix equal volume of peptide and antibody at the required dilution and leave at ambient temperature for 20 minutes. Then incubate and develop in parallel two blots: one with the antibody and one with the antibody which has been pre-absorbed with the provided peptide.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Osteopontin peptide is supplied as 100 µg of dried peptide. When the peptide is reconstituted in 200 µL water, the concentration will be 0.5 mg/mL.
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Storage
- -20 °C
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Storage Comment
- Store at -20°C. When reconstituted minimize freezing and thawing.
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