MSH alpha Peptide
Host: Synthetic
BP
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- Target See all MSH alpha products
- MSH alpha
- Peptide Type
- Synthetic
- Source
- Synthetic
- Application
- Blocking Peptide (BP)
- Purpose
- A Ligand of Melanocortin G-Protein Coupled Receptor
- Sequence
- SYSMEHFRWG KPV-NH2
- Specificity
- Toxin target: Melanocortin receptor, Ser1- Acetylation Val13- C-terminal amidation
- Characteristics
- A Ligand of Melanocortin G-Protein Coupled Receptor
- Purity
- ≥98 % (HPLC)
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- Application Notes
- Antigen Preadsorption Control: 30 - 300 pM
- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Centrifuge the vial (10,000 x g for 5 minutes) before adding solvent to spin down all the powder to the bottom of the vial. The lyophilized product may be difficult to visualize. Add solvent directly to the centrifuged vial. Gently tap, tilt, and roll the vial to aid dissolution. Avoid vigorous vortexing, light vortexing for up to 3 seconds is acceptable if needed. The product is soluble in pure water at high micromolar concentrations (100 μM - 1 mM). For long-term storage in solution, we recommend preparing a stock solution by dissolving the product in double-distilled water (ddH2O) at a concentration between 100-1000x of the final working concentration. Divide the stock solution into small aliquots and store at -20 °C. Before use, thaw the relevant vial(s) and dilute to the desired working concentration in your working buffer. Centrifuge all product preparations before use. It is recommended to prepare fresh solutions in working buffers just before use. Avoid multiple freeze-thaw cycles to maintain biological activity.
- Buffer
- Lyophilized from double distilled water (ddH2O). May contain TFA as a residual counter ion.
- Storage
- 4 °C,-20 °C
- Storage Comment
- The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.,The reconstituted solution can be stored at 4°C for up to 1 week. For longer periods (up to 6 months), small aliquots should be stored at -20°C. We do not recommend storing the product in working solutions for longer than a few days. Avoid multiple freeze-thaw cycles.
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- Target
- MSH alpha
- Background
- α-Melanotropin, alpha-MSH,α-MSH is a neuropeptide originally isolated from the pituitary gland1. α-MSH is produced by post-translational processing of a precursor protein, proopiomelanocortin (POMC)2. In most vertebrates but not in mammals, α-MSH is produced in the intermediate lobe of the pituitary gland. The biological activities of α-MSH are mediated through a family of five specific G-protein coupled receptors: MCR1, MCR2, MCR3, MCR4, and MCR5. α-MSH is an evolutionarily highly conserved peptide action that induces pigment dispersion in skin melanocytes of amphibians, reptiles and mammals by stimulating melanin production3,4.However, in human and other mammals, α-MSH acts in the brain in appetite suppression and sexual arousal. Some cases of extreme obesity have been traced to mutated α-MSH receptor in the brain. Presumably, these people are unable to respond to the appetite-suppressing effect of α-MSH5. α-MSH has significant anti-inflammatory properties, mediated through its binding to MCR16 and includes regulation of expression and secretion of chemokines, downregulation of proinflammatory signal-induced NF-kB activation and adhesion molecule expression, prostaglandin E2 synthesis, as well as induction of interleukin-107.
- Molecular Weight
- 1665 Da
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