Peptidylprolyl Cis/trans Isomerase, NIMA-Interacting 1 (PIN1) (AA 22-255), (Extracellular Domain) Peptide
PIN1
Reactivity: Human
Host: Escherichia coli (E. coli)
SDS
Dithiothreitol (DTT)
1 image
Catalog No. ABIN934902
Quick Overview for Peptidylprolyl Cis/trans Isomerase, NIMA-Interacting 1 (PIN1) (AA 22-255), (Extracellular Domain) Peptide (ABIN934902)
Target
PIN1
(Peptidylprolyl Cis/trans Isomerase, NIMA-Interacting 1 (PIN1))
Origin
Human
Source
Escherichia coli (E. coli)
Application
SDS-PAGE (SDS)
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Peptide Type
- Recombinant
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Biological Activity
- Active
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Protein Region
- AA 22-255, Extracellular Domain
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Sequence
- MADEEKLPPG WEKRMSRSSG RVYYFNHITN ASQWERPSGN SSSGGKNGQG EPARVRCSHL LVKHSQSRRP SSWRQEKITR TKEEALELIN GYIQKIKSGE EDFESLASQF SDCSSAKARG DLGAFSRGQM QKPFEDASFA LRTGEMSGPV FTDSGIHIIL RTE
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Characteristics
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Purified recombinant Human Pin 1 protein
Expression System: E.coli
Bioactivity: Specific activity is > 162 nM/min/µg, and is defined as the amount of enzyme that cleaves 1 uM of suc-AAFP-pNA per minute at 1 °C in Tris-Hcl pH 8.0 using chymotrypsin.
Molecular weight on SDS-PAGE will appear higher. -
Purity
- > 95 % pure
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Endotoxin Level
- < 1.0 EU per µg of protein (determined by LAL method)
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Peptidylprolyl Cis/trans Isomerase, NIMA-Interacting 1 (PIN1) peptide
PIN1 Reactivity: Human, Rat, Mouse, Dog Host: Synthetic BP, Imm
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Application Notes
- Pin1 protein has been used in SDS PAGE and may be suitable for use in other assays to be determined by the end user.
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Assay Procedure
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- Prepare assay buffer in a suitable container and pre-chill on ice before use: The final concentrations are 35 mM Tris, pH 8.0, 1 µM suc-AAFP-pNA).
2. Add recombinant Pin 1 protein at various concentrations (1 µg, 1 µg, 1 µg) in assay buffer.
3. Mix by inversion and equilibrate to 1 °C and monitor the A410 nm until the value is constant using a spectrophotometer.
4. Add pre-chilled chymotrypsin to 12.5 µM and mix immediately.
5. Record the increase in A410 nm for 20 minutes.
- Prepare assay buffer in a suitable container and pre-chill on ice before use: The final concentrations are 35 mM Tris, pH 8.0, 1 µM suc-AAFP-pNA).
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 1 mg/mL
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Buffer
- Supplied as a liquid in 20 mM Tris-HCl buffer, pH7.5, containing 0.1 M NaCl, 0 mM DTT, and 20 % glycerol.
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Preservative
- Dithiothreitol (DTT)
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Precaution of Use
- This product contains Dithiothreitol: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
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Handling Advice
- Avoid repeated freeze/thaw cycles.
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Storage
- RT/-20 °C
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Storage Comment
- Store at 4 °C for short term storage (1/2 weeks). Aliquot and store at -20 °C or - 70 °C for long term storage.
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- PIN1 (Peptidylprolyl Cis/trans Isomerase, NIMA-Interacting 1 (PIN1))
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Background
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Human Pin1 is a peptidyl-prolyl cis/trans isomerase (PPIase) that interacts with NIMA and essential for cell cycle regulation. Pin1 is nuclear PPIase containing a WW protein interaction domain, and is structurally and functionally related to Ess1/Ptf1, an essential protein in budding yeast. PPIase activity is necessary for Ess1/Pin1 function in yeast. Pin1 is thus an essential PPIase that regulates mitosis presumably by interacting with NIMA and attenuating its mitosis-promoting activity. Substrates of Pin1 include the mitotic regulators (Cdc25 phosphatase and NIMA ,PLK I, Wee, and Myt1 kinases), several transcription factors like beta-Catenin, c-Jun, and the tumor suppressor protein p53 , and some specific proteins like the RNA Pol II, the cytoskeleton protein tau, and the G1/S protein Cyclin D1.
Alternative Names: Rotamase Pin1 protein, UBL5 protein, PPIase protein, Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1 protein, Pin -1 protein, Pin -1, Pin 1, PPIase Pin1 protein, Pin 1 protein, PPIase Pin1 protein, Rotamase Pin1 protein, DOD protein, Peptidyl-prolyl cis-trans isomerase NIMA-interacting 1, PIN1 protein, PIN1 protein, EC 5.2.1.8 protein, Pin 1, EC 5.2.1.8 protein -
Molecular Weight
- 18.2 kDa (163 AA)
Target
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