Catechol-O-Methyltransferase (COMT) (AA 51-271) Peptide
COMT
Reactivity: Human
Host: Escherichia coli (E. coli)
SDS
1 image
Catalog No. ABIN935019
Quick Overview for Catechol-O-Methyltransferase (COMT) (AA 51-271) Peptide (ABIN935019)
Target
COMT
(Catechol-O-Methyltransferase (COMT))
Origin
Human
Source
Escherichia coli (E. coli)
Application
SDS-PAGE (SDS)
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Peptide Type
- Recombinant
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Protein Region
- AA 51-271
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Sequence
- MGDTKEQRIL NHVLQHAEPG NAQSVLEAID TYCEQKEWAM NVGDKKGKIV DAVIQEHQPS VLLELGAYCG YSAVRMARLL SPGARLITIE INPDCAAITQ RMVDFAGVKD KVTLVVGASQ DIIPQLKKKY DVDTLDMVFL DHWKDRYLPD TLLLEECGLL RKGTVLLADN VICPGAPDFL AHVRGSSCFE CTHYQSFLEY REVVDGLEKA IYKGPGSEAG P
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Characteristics
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Purified recombinant Human COMT protein
Expression System: E.coli
Molecular weight on SDS-PAGE will appear higher. -
Purity
- > 95 % pure
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Endotoxin Level
- < 1.0 EU per µg of protein (determined by LAL method)
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Application Notes
- COMT protein has been used in SDS PAGE and may be suitable for use in other assays to be determined by the end user.
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 1 mg/mL
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Buffer
- Supplied as a liquid in 20 mM Tris-HCl buffer, pH 8.0, containing 0 mM MgCl2, and 10 % glycerol.
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Handling Advice
- Avoid repeated freeze/thaw cycles.
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Storage
- RT/-20 °C
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Storage Comment
- Store at 4 °C for short term storage (1/2 weeks). Aliquot and store at -20 °C or - 70 °C for long term storage.
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- COMT (Catechol-O-Methyltransferase (COMT))
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Background
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Catechol-O-methyltrasferase (COMT) is an enzyme that catalyses the transfer of the methyl group of S-adenosylmethionine (SAM) into catechol substrates such as levodopa, dopamine, epinephrine, and norepinephirine in the presence of Mg2+. COMT appears to be located in the postsynaptic neuron and plays roles in the metabolism of catechol estrogens and the inactivation of catecholamine neurotransmitters though enzymatic degradation. COMT inhibitors prevent levodopa degradation, increase its availability and are used in the treatment of patients with Parkinson's disease. Recombinant human COMT protein was expressed in E. coli and purified by using conventional chromatography techniques.
Alternative Names: Catechol-O-methyltransferase protein, COMT, Catechol-O-methyltransferase protein -
Molecular Weight
- 24.4 kDa (221 AA)
Target
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