Aldehyde Dehydrogenase 2 Family (Mitochondrial) (ALDH2) (AA 18-517) Peptide
ALDH2
Reactivity: Human
Host: Escherichia coli (E. coli)
SDS
Dithiothreitol (DTT)
1 image
Catalog No. ABIN935020
Quick Overview for Aldehyde Dehydrogenase 2 Family (Mitochondrial) (ALDH2) (AA 18-517) Peptide (ABIN935020)
Target
ALDH2
(Aldehyde Dehydrogenase 2 Family (Mitochondrial) (ALDH2))
Origin
Human
Source
Escherichia coli (E. coli)
Application
SDS-PAGE (SDS)
-
-
Peptide Type
- Recombinant
-
Biological Activity
- Active
-
Protein Region
- AA 18-517
-
Sequence
- MSAAATQAVP APNQQPEVFC NQIFINNEWH DAVSRKTFPT VNPSTGEVIC QVAEGDKEDV DKAVKAARAA FQLGSPWRRM DASHRGRLLN RLADLIERDR TYLAALETLD NGKPYVISYL VDLDMVLKCL RYYAGWADKY HGKTIPIDGD FFSYTRHEPV GVCGQIIPWN FPLLMQAWKL GPALATGNVV VMKVAEQTPL TALYVANLIK EAGFPPGVVN IVPGFGPTAG AAIASHEDVD KVAFTGSTEI GRVIQVAAGS SNLKRVTLEL GGKSPNIIMS DADMDWAVEQ AHFALFFNQG QCCCAGSRTF VQEDIYDEFV ERSVARAKSR VVGNPFDSKT EQGPQVDETQ FKKILGYINT GKQEGAKLLC GGGIAADRGY FIQPTVFGDV QDGMTIAKEE IFGPVMQILK FKTIEEVVGR ANNSTYGLAA AVFTKDLDKA NYLSQALQAG TVWVNCYDVF GAQSPFGGYK MSGSGRELGE YGLQAYTEVK TVTVKVPQKN S
-
Characteristics
-
Purified recombinant Human ALDH2 protein
Expression System: E.coli
Bioactivity: Specific activity is < 0.2 units/mL, and was obtained by measuring the increase of NADP in absorbance at 340 nm resulting from the reduction of NAD. One unit will oxidize 1.0 uM of acetaldehyde to acetic acid per minute at pH 8.0 at 25 °C in the presence of beta-NAD, potassium and thiols.
Molecular weight on SDS-PAGE will appear higher. -
Purity
- > 90 % pure
-
Endotoxin Level
- < 1.0 EU per µg of protein (determined by LAL method)
-
-
-
-
Application Notes
- ALDH2 protein has been used in SDS PAGE and may be suitable for use in other assays to be determined by the end user.
-
Assay Procedure
-
- Prepare a 3 mL reaction mixture into a suitable container: The final concentrations are 103 mM Tris, 0.67 mM beta-NAD, 100 mM potassium chloride, 10 mM 2-mercaptoethanol, 2 mM acetaldehyde, 0.0007 % (w/v) BSA.
2. Equilibrate to 25 °C and monitor at A340 nm until value is constant using a spectrophotometer.
3. Add 5 µg of recombinant ALDH2 into reaction mixture and mix immediately.
4. Record the increase at A340 nm for 5 minutes
- Prepare a 3 mL reaction mixture into a suitable container: The final concentrations are 103 mM Tris, 0.67 mM beta-NAD, 100 mM potassium chloride, 10 mM 2-mercaptoethanol, 2 mM acetaldehyde, 0.0007 % (w/v) BSA.
-
Restrictions
- For Research Use only
-
-
-
Format
- Liquid
-
Concentration
- 1 mg/mL
-
Buffer
- Supplied as a liquid in 20 mM Tris-HCl buffer, pH 7.5, containing 0 mM DTT, 0 mM EDTA, and 10 % glycerol.
-
Preservative
- Dithiothreitol (DTT)
-
Precaution of Use
- This product contains Dithiothreitol: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.
-
Handling Advice
- Avoid repeated freeze/thaw cycles.
-
Storage
- RT/-20 °C
-
Storage Comment
- Store at 4 °C for short term storage (1/2 weeks). Aliquot and store at -20 °C or - 70 °C for long term storage.
-
-
- ALDH2 (Aldehyde Dehydrogenase 2 Family (Mitochondrial) (ALDH2))
-
Background
-
ALDH2 (Aldehyde dehydrogenase 2 family) belongs to the aldehyde dehydrogenase family which catalyze the chemical transformation from acetaldehyde to acetic acid and is the second enzyme of the major oxidative pathway of alcohol metabolism. There are two major liver isoforms of this enzyme, cytosolic and mitochondrial, and they can be also distinguished by their electrophoretic mobilities, kinetic properties, and subcellular localizations. Recombinant human ALDH2 protein was expressed in E. coli and purified by using conventional chromatography techniques.
Alternative Names: Aldehyde dehydrogenase mitochondrial protein, ALDH E2 protein, mitochondrial Acetaldehyde dehydrogenase 2 protein, ALDH-2, Aldehyde dehydrogenase protein, ALDH 2 protein, ALDH 2 protein, ALDH-2 protein, ALDM protein, MGC1806 protein, ALDH 2, Aldehyde dehydrogenase 2 family protein, Mitochondrial aldehyde dehydrogenase 2 protein, ALDH class 2 protein, ALDH2, ALDHI protein, Nucleus encoded mitochondrial aldehyde dehydrogenase 2., Aldehyde dehydrogenase 2 family (mitochondrial) protein, ALDH-E2 protein, Liver mitochondrial ALDH protein -
Molecular Weight
- 54.5 kDa (501 AA)
Target
-
You are here:
