Polymerase (RNA) III (DNA Directed) Polypeptide A, 155kDa (POLR3A) Peptide
POLR3A
Reactivity: Mammalian
Host: Synthetic
BP, WB, IHC
Catalog No. ABIN937013
Quick Overview for Polymerase (RNA) III (DNA Directed) Polypeptide A, 155kDa (POLR3A) Peptide (ABIN937013)
Target
POLR3A
(Polymerase (RNA) III (DNA Directed) Polypeptide A, 155kDa (POLR3A))
Origin
Mammalian
Source
Synthetic
Application
Blocking Peptide (BP), Western Blotting (WB), Immunohistochemistry (IHC)
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Peptide Type
- Synthetic
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Sequence
- AYFGQKDSVC GVSECIIMGI PMNIGTGLFK LLHKADRDPN PPKRPLIFDT
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Characteristics
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A synthetic peptide for use as a blocking control in assays to test for specificity of POLR3 A antibody,
Alternative Names: POLR3A control peptide, POLR3A antibody Blocking Peptide, Anti-POLR3A Blocking Peptide, RNA Polymerase IIIA Blocking Peptide, RPC1 Blocking Peptide, RPC155 Blocking Peptide, hRPC155 Blocking Peptide, POLR3A, POLRA-3, POLRA 3, POLRA-3 Blocking Peptide, POLRA 3 Blocking Peptide
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Application Notes
- Optimal conditions should be determined by the investigator
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Add 100 µL of distilled water for a final peptide concentration is 1 mg/mL.
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Buffer
- PBS
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Handling Advice
- Avoid repeated freeze/thaw cycles.
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Storage
- -20 °C
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Storage Comment
- Store at -20 °C long term.
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- POLR3A (Polymerase (RNA) III (DNA Directed) Polypeptide A, 155kDa (POLR3A))
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Background
- DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. POLR3A is the largest and catalytic core component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. It forms the polymerase active center together with the second largest subunit. A single stranded DNA template strand of the promoter is positioned within the central active site cleft of Pol III. A bridging helix emanates from RPC1 and crosses the cleft near the catalytic site and is thought to promote translocation of Pol III by acting as a ratchet that moves the RNA-DNA hybrid through the active site by switching from straight to bent conformations at each step of nucleotide addition.
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Molecular Weight
- 156 kDa
Target
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