Protein Inhibitor of Activated STAT, 2 (PIAS2) (N-Term) Peptide
PIAS2
Reactivity: Human, Mouse
Host: Synthetic
BP, WB
Catalog No. ABIN981731
Quick Overview for Protein Inhibitor of Activated STAT, 2 (PIAS2) (N-Term) Peptide (ABIN981731)
Target
PIAS2
(Protein Inhibitor of Activated STAT, 2 (PIAS2))
Origin
Human, Mouse
Source
Synthetic
Application
Blocking Peptide (BP), Western Blotting (WB)
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Protein Region
- N-Term
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Sequence
- PAVQIKIREL YRRRYPRTLE GLCDLSTIKS SVFSLDGSSS PVEPDLPVAG
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Characteristics
- This is a synthetic peptide designed for use in combination with anti-Pias2 Antibody. It may block above mentioned antibody from binding to its target protein in western blot and/or immunohistochecmistry under proper experimental settings. There is no guarantee for its use in other applications.
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Purification
- Purified
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Application Notes
- Each Investigator should determine their own optimal working dilution for specific applications.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Add 100 μL of sterile PBS. Final peptide concentration is 1 mg/mL in PBS.
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Concentration
- 1 mg/mL
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Buffer
- Final peptide concentration is 1 mg/mL in PBS.
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Handling Advice
- Avoid repeated freeze-thaw cycles.
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Storage
- -20 °C
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Storage Comment
- For longer periods of storage, store at -20°C. Avoid repeat freeze-thaw cycles.
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- PIAS2 (Protein Inhibitor of Activated STAT, 2 (PIAS2))
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Background
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Pias2 functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor.Pias2 plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway, the p53 pathway and the steroid hormone signaling pathway. The effects of this transcriptional coregulation, transactivation or silencing may vary depending upon the biological context and PIAS2 isoform studied. However, it seems to be mostly involved in gene silencing.Pias2 binds to sumoylated ELK1 and enhances its transcriptional activity by preventing recruitment of HDAC2 by ELK1, thus reversing SUMO-mediated repression of ELK1 transactivation activity. Isoform PIASx-beta, but not isoform PIASx-alpha, promotes MDM2 sumoylation. Isoform PIASx-alpha promotes PARK7 sumoylation. Isoform PIASx-beta promotes NCOA2 sumoylation more efficiently than isoform PIASx-alpha
Alias Symbols: 6330408K17Rik, AI462206, ARIP3, AU018068, Dib, Miz1, PIASxalpha, PIASxb, PIASxbeta, SIZ2
Protein Interaction Partner: Gtf2i,Hdac3,DHX9,GTF2I,GTF2IRD1,Gtf2i,HDAC3,Nfkb1,Trim32,Trp53
Protein Size: 580 -
Molecular Weight
- 63 kDa
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Gene ID
- 17344
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NCBI Accession
- NM_001164167, NP_001157639
Target
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