Nuclear Factor-kB p65 (NFkBP65) (Middle Region) Peptide
NFkBP65
Reactivity: Human
Host: Synthetic
BP, WB
Catalog No. ABIN982837
Quick Overview for Nuclear Factor-kB p65 (NFkBP65) (Middle Region) Peptide (ABIN982837)
Target
NF-kB p65 (NFkBP65)
(Nuclear Factor-kB p65 (NFkBP65))
Origin
Human
Source
Synthetic
Application
Blocking Peptide (BP), Western Blotting (WB)
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Protein Region
- Middle Region
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Characteristics
- This is a synthetic peptide designed for use in combination with anti-RELA antibody (Catalog #: ARP30371_P050). It may block above mentioned antibody from binding to its target protein in western blot and/or immunohistochecmistry under proper experimental settings. There is no guarantee for its use in other applications.
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Purification
- Purified
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Nuclear Factor-kB p65 (NFkBP65) peptide
NFkBP65 Host: Synthetic Sodium azide
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Application Notes
- Each Investigator should determine their own optimal working dilution for specific applications.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Add 100 μL of sterile PBS. Final peptide concentration is 1 mg/mL in PBS.
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Concentration
- 1 mg/mL
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Buffer
- Final peptide concentration is 1 mg/mL in PBS.
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Handling Advice
- Avoid repeated freeze-thaw cycles.
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Storage
- -20 °C
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Storage Comment
- For longer periods of storage, store at -20°C. Avoid repeat freeze-thaw cycles.
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- NF-kB p65 (NFkBP65) (Nuclear Factor-kB p65 (NFkBP65))
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Background
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P65 is a subunit of the nuclear factor kappa-B, a second messenger, which activates the transcription of a number of genes in multiple tissues. The inhibitory effect of I-kappa-B upon NF-kappa-B in the cytoplasm is exerted primarily through the interaction with p65. p65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kappa-B complex. NFKB1 (MIM 164011) or NFKB2 (MIM 164012) is bound to REL (MIM 164910), RELA, or RELB (MIM 604758) to form the NFKB complex. The p50 (NFKB1)/p65 (RELA) heterodimer is the most abundant form of NFKB. The NFKB complex is inhibited by I-kappa-B proteins (NFKBIA, MIM 164008 or NFKBIB, MIM 604495), which inactivate NFKB by trapping it in the cytoplasm. Phosphorylation of serine residues on the I-kappa-B proteins by kinases (IKBKA, MIM 600664, or IKBKB, MIM 603258) marks them for destruction via the ubiquitination pathway, thereby allowing activation of the NFKB complex. Activated NFKB complex translocates into the nucleus and binds DNA at kappa-B-binding motifs such as 5-prime GGGRNNYYCC 3-prime or 5-prime HGGARNYYCC 3-prime (where H is A, C, or T, R is an A or G purine, and Y is a C or T pyrimidine).[supplied by OMIM]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications. PRIMARYREFSEQ_SPAN PRIMARY_IDENTIFIER PRIMARY_SPAN COMP 1-1760 M62399.1 8-1767
Alias Symbols: MGC131774, NFKB3, p65
Protein Interaction Partner: AES,BIRC3,CARM1,CCND1,CHEK1,CHUK,CREBBP,CREBBP,CXCL10,EGR1,EP300,EP300,FOS,IL8,ING4,JUN,NCOA3,NFKB1,NFKB1,NFKBIA,NFKBIA,NFKBIB,NFKBIB,SLC1A2,TERT,TLE1,AATF,ACTL6A,AES,AHR,AKAP8,APBA2,AR,BRCA1,BTK,BTRC,CALM1,CAMK4,CARM1,CDK9,CEBPB,CEBPD,CHEK1,CHUK,COMMD1,CREBBP,CSNK2A1,DDX1,DHX9,EGR1,EP300,ESR1,ETHE1,FAF1,FOS,FUS,GTF2B,HDAC1,HDAC2,HDAC3,HEXIM1,HMGA2,HMGB1,IKBKB,ING4,IRAK1BP1,IRF1,IRF2,IRF3,JUN,KAT2B,KPNA2,MAP2K6,MAP3K7,MAP3K8,MEN1,MST1R,MTPN,MYC,NCOA3,NCOR2,NFKB1,NFKB2,NFKBIA,NFKBIB,NFKBIE,NKRF,NKX2-1,NOTCH1,NR3C1,PARP1,PDCD11,PGR,PIAS1,PIAS3,PIN1,PML,POU2F1,PPARA,PPP1R13L,PPP2CA,PPP2R1B,PPP4C,PRKACA,PRKCZ,PRTN3,REL,RELA,RELB,REPS2,RFC1,RNF25,RPS6KA5,RXRA,SIRT1,SNIP1,SOCS1,SP1,SRF,STAT1,STAT3,STAT6,TAF1,TAF11,TAF4B,TAF6,TBK1,TBP,TERT,TNIP2,TP53BP1,TP53BP2,TRIB3,TRIP4,TSC22D3,TWIST1,UNC5CL,AES,AHR,AKAP8L,APBA2,ASCC1,ASCC2,ASCC3,BARD1,BRCA1,BTRC,CDK9,CEBPB,CEBPD,COMMD1,CREBBP,CSNK2A1,CSNK2A2,DHX9,EGR1,EP300,ETHE1,FOS,FUS,GTF2B,HDAC1,HDAC2,HDAC3,HEXIM1,HMGA2,HMGB1,Hdac1,ING4,IQGAP2,IRF2,IRF8,IRF9,ITGB3BP,JUN,KDM2A,KPNA2,MAP3K8,MEN1,MTPN,MYC,NCF1,NCOA6,NCOR2,NFKB1,NFKB2,NFKBIA,NFKBIB,NFKBIE,NKRF,NKX2-1,NOTCH1,NR3C1,NSD1,PARP1,PDLIM2,PGR,PIAS3,PML,POU2F1,PPARA,PPP1R13L,PPP2CA,PPP2CB,PPP4C,PRKCZ,REL,RELA,RELB,RFC1,RNF25,RXRA,SOCS1,SP1,SRF,STAT3,TAF1,TAF11,TAF4B,TAF6,TBK1,TBP,TERT,TLE1,TNIP2,TRIB3,TWIST1,UBC,UNC5CL
Protein Size: 551 -
Molecular Weight
- 60 kDa
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Gene ID
- 5970
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NCBI Accession
- NM_021975, NP_068810
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UniProt
- Q04206
Target
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