Topoisomerase (DNA) II beta 180kDa (TOP2B) (Middle Region) Peptide
TOP2B
Reactivity: Human
Host: Synthetic
BP, WB
Catalog No. ABIN985375
Quick Overview for Topoisomerase (DNA) II beta 180kDa (TOP2B) (Middle Region) Peptide (ABIN985375)
Target
TOP2B
(Topoisomerase (DNA) II beta 180kDa (TOP2B))
Origin
Human
Source
Synthetic
Application
Blocking Peptide (BP), Western Blotting (WB)
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Protein Region
- Middle Region
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Characteristics
- This is a synthetic peptide designed for use in combination with anti-TOP2B antibody (Catalog #: ARP33079_P050). It may block above mentioned antibody from binding to its target protein in western blot and/or immunohistochecmistry under proper experimental settings. There is no guarantee for its use in other applications.
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Purification
- Purified
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Application Notes
- Each Investigator should determine their own optimal working dilution for specific applications.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- Add 100 μL of sterile PBS. Final peptide concentration is 1 mg/mL in PBS.
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Concentration
- 1 mg/mL
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Buffer
- Final peptide concentration is 1 mg/mL in PBS.
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Handling Advice
- Avoid repeated freeze-thaw cycles.
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Storage
- -20 °C
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Storage Comment
- For longer periods of storage, store at -20°C. Avoid repeat freeze-thaw cycles.
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- TOP2B (Topoisomerase (DNA) II beta 180kDa (TOP2B))
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Background
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TOP2B is a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also play a role in ataxia-telangiectasia. This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, beta, is localized to chromosome 3 and the alpha form is localized to chromosome 17. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also play a role in ataxia-telangiectasia. Alternative splicing of this gene results in two transcript variants, however, the second variant has not yet been fully described. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
Alias Symbols: TOPIIB, top2beta
Protein Interaction Partner: MAPK3,CD3E,CSNK2B,HDAC1,MAPK3,SUMO1,TOP2A,TOP2B,TOPBP1,TP53,XPO1,XRCC5,BAZ1B,CD3E,CSNK2B,HDAC1,HDAC2,MTA2,SUMO1,TOP2A,TOPBP1,TP53
Protein Size: 1621 -
Molecular Weight
- 183 kDa
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Gene ID
- 7155
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NCBI Accession
- NM_001068, NP_001059
Target
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