We are preparing the requested document. Please wait, this may take a while...!
|Antigen||HLA-DR-gamma (CD74) Antibodies|
|Reactivity||Baboon, Human, Mouse (Murine) Alternatives|
|Conjugate||This CD74 antibody is un-conjugated Alternatives|
Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (IHC), Staining Methods (StM)
Product Details anti-CD74 AntibodyTarget Details CD74 Application Details Handling Images
|Specificity||It recognizes a protein of ~35 kDa, identified as CD74 (Workshop IV). CD74 is a type II transmembrane protein which binds to the peptide binding groove of newly synthesized MHC class II alpha/beta heterodimers and prevents their premature association with endogenous polypeptides. CD74 is expressed primarily by antigen presenting cells, such as B-lymphocytes (from before the pre-B cell stage to before the plasma cell stage), macrophages, and monocytes, and many epithelial cells. Anti-CD74 stains predominantly germinal center lymphocytes and B-cell lymphomas, but rarely T-cell lymphomas. Anti-CD74 has been shown to be useful in differentiating atypical fibroxanthoma (-) from malignant fibrous histiocytoma (+).|
|No Cross-Reactivity||Rat (Rattus)|
|Purification||Purified by Protein A/G|
|Immunogen||SU-DHL-4 lymphoma cells|
|Plasmids, Primers & others|
Target Details CD74Product Details anti-CD74 Antibody Application Details Handling Images back to top
|Alternative Name||CD74 (CD74 Antibody Abstract)|
|Pathways||Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Negative Regulation of intrinsic apoptotic Signaling, Cancer Immune Checkpoints|
Application DetailsProduct Details anti-CD74 Antibody Target Details CD74 Handling Images back to top
Positive Control: Daudi or Raji cells. Tonsil or lymph node.
Known Application: Flow Cytometry (0.5-1 μg/million cells), (Methanol Fixed)Immunofluorescence (1-4 μg/mL), Immunohistochemistry (Formalin-fixed) (1-2 μg/mL for 30 minutes at RT)(Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20 minutes)Optimal dilution for a specific application should be determined.
|Restrictions||For Research Use only|
HandlingProduct Details anti-CD74 Antibody Target Details CD74 Application Details Images back to top
|Buffer||10 mM PBS with 0.05 % BSA & 0.05 % azide.|
|Precaution of Use||This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.|
|Storage||4 °C,-80 °C|
|Storage Comment||Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.|
|Expiry Date||24 months|