Cathepsin D Protein (CTSD) (AA 67-403, partial) (GST tag)

Catalog No. ABIN1095534

This Recombinant Cathepsin D protein is produced in Escherichia coli (E. coli). There are 3 publications available.

Quick Overview for Cathepsin D Protein (CTSD) (AA 67-403, partial) (GST tag) (ABIN1095534)

Target: Cathepsin D (CTSD)

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: ELISA

Purity: 90 %

Product Details

Protein Characteristics: AA 67-403, partial

Purification tag / Conjugate: This Cathepsin D protein is labelled with GST tag.

Sequence: IPEVLKNYMD AQYYGEIGIG TPPQCFTVVF DTGSSNLWVP SIHCKLLDIA CWIHHKYNSD KSSTYVKNGT SFDIHYGSGS LSGYLSQDTV SVPCQSASSA SALGGVKVER QVFGEATKQP GITFIAAKFD GILGMAYPRI SVNNVLPVFD NLMQQKLVDQ NIFSFYLSRD PDAQPGGELM LGGTDSKYYK GSLSYLNVTR KAYWQVHLDQ VEVASGLTLC KEGCEAIVDT GTSLMVGPVD EVRELQKAIG AVPLIQGEYM IPCEKVSTLP AITLKLGGKG YKLSPEDYTL KVSQAGKTLC LSGFMGMDIP PPSGPLWILG DVFIGRYYTV FDRDNNR

Characteristics: Please inquire if you are interested in this recombinant protein expressed in E. coli, mammalien cells or by baculovirus infection. Be aware about differences in price and lead time.

Application Details

Comment:

The yeast protein expression system is the most economical and efficient eukaryotic system for secretion and intracellular expression. A protein expressed by the mammalian cell system is of very high-quality and close to the natural protein. But the low expression level, the high cost of medium and the culture conditions restrict the promotion of mammalian cell expression systems. The yeast protein expression system serve as a eukaryotic system integrate the advantages of the mammalian cell expression system. A protein expressed by yeast system could be modificated such as glycosylation, acylation, phosphorylation and so on to ensure the native protein conformation. It can be used to produce protein material with high added value that is very close to the natural protein. Our proteins produced by yeast expression system has been used as raw materials for downstream preparation of monoclonal antibodies.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: 0.2-2 mg/mL

Buffer: Tris-based buffer, 50 % glycerol

Handling Advice: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week

Storage: -20 °C

Storage Comment: Store at -20 °C for extended storage, conserve at -20 °C or -80 °C

References

Redecker, Heckendorf, Grosch, Mersmann, Hasilik: "Molecular organization of the human cathepsin D gene." in: DNA and cell biology, Vol. 10, Issue 6, pp. 423-31, (1991) (PubMed).

Westley, May: "Oestrogen regulates cathepsin D mRNA levels in oestrogen responsive human breast cancer cells." in: Nucleic acids research, Vol. 15, Issue 9, pp. 3773-86, (1987) (PubMed).

Faust, Kornfeld, Chirgwin: "Cloning and sequence analysis of cDNA for human cathepsin D." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 82, Issue 15, pp. 4910-4, (1985) (PubMed).

Target Details

Target: Cathepsin D (CTSD)

Alternative Name: Cathepsin D protein

Background: Acid protease active in intracellular protein breakdown. Involved in the pathogenesis of several diseases such as breast cancer and possibly Alzheimer disease.

Molecular Weight: 64.2 kD

UniProt: P07339

Pathways: Peptide Hormone Metabolism