PRKCSH Protein (AA 15-302, partial) (GST tag)

Catalog No. ABIN1095608

This Recombinant PRKCSH protein is expressed in Escherichia coli (E. coli) and has been mentioned in 3+ publications.

Quick Overview for PRKCSH Protein (AA 15-302, partial) (GST tag) (ABIN1095608)

Target: PRKCSH (Protein Kinase C Substrate 80K-H (PRKCSH))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: ELISA

Purity: 90 %

Product Details

Protein Characteristics: AA 15-302, partial

Purification tag / Conjugate: This PRKCSH protein is labelled with GST tag.

Sequence: VEVKRPRGVS LTNHHFYDES KPFTCLDGSA TIPFDQVNDD YCDCKDGSDE PGTAACPNGS FHCTNTGYKP LYIPSNRVND GVCDCCDGTD EYNSGVICEN TCKEKGRKER ESLQQMAEVT REGFRLKKIL IEDWKKAREE KQKKLIELQA GKKSLEDQVE MLRTVKEEAE KPEREAKEQH QKLWEEQLAA AKAQQEQELA ADAFKELDDD MDGTVSVTEL QTHPELDTDG DGALSEAEAQ ALLSGDTQTD ATSFYDRVWA AIRDKYRSEA LPTDLPAPSA PDLTEPKE

Characteristics: Please inquire if you are interested in this recombinant protein expressed in E. coli, mammalien cells or by baculovirus infection. Be aware about differences in price and lead time.

Application Details

Comment:

The yeast protein expression system is the most economical and efficient eukaryotic system for secretion and intracellular expression. A protein expressed by the mammalian cell system is of very high-quality and close to the natural protein. But the low expression level, the high cost of medium and the culture conditions restrict the promotion of mammalian cell expression systems. The yeast protein expression system serve as a eukaryotic system integrate the advantages of the mammalian cell expression system. A protein expressed by yeast system could be modificated such as glycosylation, acylation, phosphorylation and so on to ensure the native protein conformation. It can be used to produce protein material with high added value that is very close to the natural protein. Our proteins produced by yeast expression system has been used as raw materials for downstream preparation of monoclonal antibodies.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: 0.2-2 mg/mL

Buffer: Tris-based buffer, 50 % glycerol

Handling Advice: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week

Storage: -20 °C

Storage Comment: Store at -20 °C for extended storage, conserve at -20 °C or -80 °C

References

Pelletier, Marcil, Sevigny, Jakob, Tessier, Chevet, Menard, Bergeron, Thomas: "The heterodimeric structure of glucosidase II is required for its activity, solubility, and localization in vivo." in: Glycobiology, Vol. 10, Issue 8, pp. 815-27, (2000) (PubMed).

Ophoff, Terwindt, Vergouwe, van Eijk, Mohrenweiser, Litt, Hofker, Haan, Ferrari, Frants: "A 3-Mb region for the familial hemiplegic migraine locus on 19p13.1-p13.2: exclusion of PRKCSH as a candidate gene. Dutch Migraine Genetic Research Group." in: European journal of human genetics : EJHG, Vol. 4, Issue 6, pp. 321-8, (1997) (PubMed).

Sakai, Hirai, Minoshima, Kudoh, Fukuyama, Shimizu: "Isolation of cDNAs encoding a substrate for protein kinase C: nucleotide sequence and chromosomal mapping of the gene for a human 80K protein." in: Genomics, Vol. 5, Issue 2, pp. 309-15, (1989) (PubMed).

Target Details

Target: PRKCSH (Protein Kinase C Substrate 80K-H (PRKCSH))

Alternative Name: Glucosidase 2 subunit beta protein

Background: Regulatory subunit of glucosidase II.

Molecular Weight: 59.6 kD

UniProt: P14314

Pathways: Cellular Glucan Metabolic Process, Methionine Biosynthetic Process