MYL6 Protein (AA 3-151, partial) (GST tag)

Catalog No. ABIN1047437

Recombinant MYL6 protein expressed in Escherichia coli (E. coli) and has been mentioned in 4+ publications.

Quick Overview for MYL6 Protein (AA 3-151, partial) (GST tag) (ABIN1047437)

Target: MYL6 (Myosin Light Chain 6, Alkali, Smooth Muscle and Non Muscle (MYL6))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: ELISA

Purity: 90 %

Product Details

Protein Characteristics: AA 3-151, partial

Purification tag / Conjugate: This MYL6 protein is labelled with GST tag.

Sequence: DFTEDQTAEF KEAFQLFDRT GDGKILYSQC GDVMRALGQN PTNAEVLKVL GNPKSDEMNV KVLDFEHFLP MLQTVAKNKD QGTYEDYVEG LRVFDKEGNG TVMGAEIRHV LVTLGEKMTE EEVEMLVAGH EDSNGCINYE AFVRHILSG

Characteristics: Please inquire if you are interested in this recombinant protein expressed in E. coli, mammalien cells or by baculovirus infection. Be aware about differences in price and lead time.

Application Details

Comment:

The yeast protein expression system is the most economical and efficient eukaryotic system for secretion and intracellular expression. A protein expressed by the mammalian cell system is of very high-quality and close to the natural protein. But the low expression level, the high cost of medium and the culture conditions restrict the promotion of mammalian cell expression systems. The yeast protein expression system serve as a eukaryotic system integrate the advantages of the mammalian cell expression system. A protein expressed by yeast system could be modificated such as glycosylation, acylation, phosphorylation and so on to ensure the native protein conformation. It can be used to produce protein material with high added value that is very close to the natural protein. Our proteins produced by yeast expression system has been used as raw materials for downstream preparation of monoclonal antibodies.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: 0.2-2 mg/mL

Buffer: Tris-based buffer, 50 % glycerol

Handling Advice: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week

Storage: -20 °C

Storage Comment: Store at -20 °C for extended storage, conserve at -20 °C or -80 °C

References

Watanabe, Kohri, Takaishi, Horie, Higashihara: "Molecular cloning and sequencing of myosin light chains in human megakaryoblastic leukemia cells." in: Journal of smooth muscle research = Nihon Heikatsukin Gakkai kikanshi, Vol. 37, Issue 1, pp. 25-38, (2001) (PubMed).

Bora, Bora, Wu, Kaplan, Lange: "Molecular cloning, sequencing, and characterization of smooth muscle myosin alkali light chain from human eye cDNA: homology with myocardial fatty acid ethyl ester synthase-III cDNA." in: Genomics, Vol. 19, Issue 1, pp. 186-8, (1994) (PubMed).

Hailstones, Gunning: "Characterization of human myosin light chains 1sa and 3nm: implications for isoform evolution and function." in: Molecular and cellular biology, Vol. 10, Issue 3, pp. 1095-104, (1990) (PubMed).

Lenz, Lohse, Seidel, Arnold: "The alkali light chains of human smooth and nonmuscle myosins are encoded by a single gene. Tissue-specific expression by alternative splicing pathways." in: The Journal of biological chemistry, Vol. 264, Issue 15, pp. 9009-15, (1989) (PubMed).

Target Details

Target: MYL6 (Myosin Light Chain 6, Alkali, Smooth Muscle and Non Muscle (MYL6))

Alternative Name: Myosin light polypeptide 6 protein

Background: Regulatory light chain of myosin. Does not bind calcium.

Molecular Weight: 44.1 kD

UniProt: P60660