Seryl-tRNA Synthetase (SARS) (AA 2-233), (partial) protein (GST tag)

Catalog No. ABIN1047631

This Recombinant protein is produced in Escherichia coli (E. coli). There are 2 publications available.

Quick Overview for Seryl-tRNA Synthetase (SARS) (AA 2-233), (partial) protein (GST tag) (ABIN1047631)

Target: Seryl-tRNA Synthetase (SARS)

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Application: ELISA

Purity: 90 %

Product Details

Protein Characteristics: AA 2-233, partial

Purification tag / Conjugate: GST tag

Sequence: VLDLDLFRVD KGGDPALIRE TQEKRFKDPG LVDQLVKADS EWRRCRFRAD NLNKLKNLCS KTIGEKMKKK EPVGDDESVP ENVLSFDDLT ADALANLKVS QIKKVRLLID EAILKCDAER IKLEAERFEN LREIGNLLHP SVPISNDEDV DNKVERIWGD CTVRKKYSHV DLVVMVDGFE GEKGAVVAGS RGYFLKGVLV FLEQALIQYA LRTLGSRGYI PIYTPFFMRK EV

Characteristics: Please inquire if you are interested in this recombinant protein expressed in E. coli, mammalien cells or by baculovirus infection. Be aware about differences in price and lead time.

Application Details

Comment:

The yeast protein expression system is the most economical and efficient eukaryotic system for secretion and intracellular expression. A protein expressed by the mammalian cell system is of very high-quality and close to the natural protein. But the low expression level, the high cost of medium and the culture conditions restrict the promotion of mammalian cell expression systems. The yeast protein expression system serve as a eukaryotic system integrate the advantages of the mammalian cell expression system. A protein expressed by yeast system could be modificated such as glycosylation, acylation, phosphorylation and so on to ensure the native protein conformation. It can be used to produce protein material with high added value that is very close to the natural protein. Our proteins produced by yeast expression system has been used as raw materials for downstream preparation of monoclonal antibodies.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Concentration: 0.2-2 mg/mL

Buffer: Tris-based buffer, 50 % glycerol

Handling Advice: Repeated freezing and thawing is not recommended. Store working aliquots at 4 °C for up to one week

Storage: -20 °C

Storage Comment: Store at -20 °C for extended storage, conserve at -20 °C or -80 °C

References

Ota, Suzuki, Nishikawa, Otsuki, Sugiyama, Irie, Wakamatsu, Hayashi, Sato, Nagai, Kimura, Makita, Sekine, Obayashi, Nishi, Shibahara, Tanaka, Ishii, Yamamoto, Saito, Kawai, Isono, Nakamura, Nagahari et al.: "Complete sequencing and characterization of 21,243 full-length human cDNAs. ..." in: Nature genetics, Vol. 36, Issue 1, pp. 40-5, (2003) (PubMed).

Vincent, Tarbouriech, Härtlein: "Genomic organization, cDNA sequence, bacterial expression, and purification of human seryl-tRNA synthase." in: European journal of biochemistry / FEBS, Vol. 250, Issue 1, pp. 77-84, (1998) (PubMed).

Target Details

Target: Seryl-tRNA Synthetase (SARS)

Alternative Name: Seryl-tRNA synthetase,cytoplasmic protein

Background: Catalyzes the attachment of serine to tRNA(Ser). Is also probably able to aminoacylate tRNA(Sec) with serine, to form the misacylated tRNA L-seryl-tRNA(Sec), which will be further converted into selenocysteinyl-tRNA(Sec).

Molecular Weight: 53.8 kD

UniProt: P49591