VIRD2 Protein (AA 1-424) (His tag)

Catalog No. ABIN1047713

This Recombinant VIRD2 protein is produced in Escherichia coli (E. coli). There are 2 publications available.

Quick Overview for VIRD2 Protein (AA 1-424) (His tag) (ABIN1047713)

Target: VIRD2 (Endonuclease, VIRD2 (VIRD2))

Protein Type: Recombinant

Origin: Rhizobium

Source: Escherichia coli (E. coli)

Application: ELISA, SDS-PAGE (SDS)

Purity: 90 %

Product Details

Protein Characteristics: AA 1-424

Purification tag / Conjugate: This VIRD2 protein is labelled with His tag.

Sequence: MPDRAQVIIR IVPGGGTKTL QQIINQLEYL SRKGKLELQR SARHLDIPVP PDQIRELAQS WVTEAGIYDE SQSDDDRQQD LTTHIIVSFP AGTDQTAAYE ASREWAAEMF GSGYGGGRYN YLTAYHVDRD HPHLHVVVNR RELLGHGWLK ISRRHPQLNY DGLRKKMAEI SLRHGIVLDA TSRAERGIAE RPITYAEHRR LERMQAQKIQ FEDTDFDETS PEEDRRDLSQ SFDPFRSDPS TGEPDRATRH DKQPLEQHAR FQESAGSSIK ADARIRVSLE SERSAQPSAS KIPVIGHFGI ETSYVAEASV RKRSGIFGTS RPVTDVAMHT VKRQQRSKRR NDEEAGPSGA NRKGLKAAQV DSEANVGEQD TRDDSNKAAD PVSASIGTEQ PEASPKRPRD RHDGELGGRK RARGNRRDDG RGGT

Characteristics: Recombinant Rhizobium radiobacter T-DNA border endonuclease virD2 (virD2) with N-terminal 10xHis-tag and C-terminal Myc-tag

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20/-80°C

Buffer: the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

Storage: -20 °C

References

Rogowsky, Powell, Shirasu, Lin, Morel, Zyprian, Steck, Kado: "Molecular characterization of the vir regulon of Agrobacterium tumefaciens: complete nucleotide sequence and gene organization of the 28.63-kbp regulon cloned as a single unit." in: Plasmid, Vol. 23, Issue 2, pp. 85-106, (1990) (PubMed).

Hagiya, Close, Tait, Kado: "Identification of pTiC58 plasmid-encoded proteins for virulence in Agrobacterium tumefaciens." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 82, Issue 9, pp. 2669-73, (1985) (PubMed).

Target Details

Target: VIRD2 (Endonuclease, VIRD2 (VIRD2))

Alternative Name: T-DNA border endonuclease virD2

Molecular Weight: 55 kDa

UniProt: P06668