beta-r2 (AA 27-243) protein (His tag)
Origin: Human
Host: Human Cells
Recombinant
> 95 % as determined by reducing SDS-PAGE.
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- Target
- beta-r2
- Protein Type
- Recombinant
- Protein Characteristics
- AA 27-243
- Origin
- Human
- Source
- Human Cells
- Purification tag / Conjugate
- His tag
- Purpose
- Recombinant Human Interferon α/β Receptor 2/IFNAR2 (C-6His)
- Sequence
- ISYDSPDYTD ESCTFKISLR NFRSILSWEL KNHSIVPTHY TLLYTIMSKP EDLKVVKNCA NTTRSFCDLT DEWRSTHEAY VTVLEGFSGN TTLFSCSHNF WLAIDMSFEP PEFEIVGFTN HINVMVKFPS IVEEELQFDL SLVIEEQSEG IVKKHKPEIK GNMSGNFTYI IDKLIPNTNY CVSVYLEHSD EQAVIKSPLK CTLLPPGQES ESAESAKVDH HHHHH
- Characteristics
- Recombinant Human Interferon alpha/beta Receptor 2/IFNAR2 produced by transfected human cells is a secreted protein with sequence (Ile27-Lys243) of Human IFNAR2 fused with a polyhistidine tag at the C-terminus.
- Purity
- > 95 % as determined by reducing SDS-PAGE.
- Sterility
- 0.2 μm filtered
- Endotoxin Level
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
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It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. - Buffer
- Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.2.
- Handling Advice
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
- Storage
- 4 °C/-20 °C/-80 °C
- Storage Comment
-
Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months. - Expiry Date
- 3 months
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- Target
- beta-r2
- Sub Type
- Fusionprotein
- Background
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Interferon alpha/beta Receptor 2 (IFN-alpha/beta R2) is a single-pass type I membrane protein which belongs to the type II cytokine receptor family. It complexes with IFN-alpha/beta R1 to form the signaling receptor complex for the family of alpha and beta IFN subtypes. By alternative splicing, IFN-alpha/beta R2 can exist as a secreted soluble protein or as a type I membrane protein. IFN-alpha/beta R2 is the principal ligand binding subunit of the receptor. Ligand binding is stabilized by the subsequent association with IFN-alpha/beta R1, resulting in the formation of a signaling ternary receptor complex. IFNAR2 was detected in most lymphocytes, monocytes, and granulocytes, although IFNAR2 expression was higher in the monocytes and granulocytes than in the lymphocytes. Among the lymphocyte subsets, IFNAR2 showed high expression in natural killer (NK) cells and low expression in T lymphocytes. Isoform 1 and isoform 3 of IFNAR2 are directly involved in signal transduction due to their interaction with the TYR kinase, JAK1. Isoform 1 also interacts with the transcriptional factors, STAT1 and STAT2. Both forms are potent inhibitors of type I IFN activity.
Alternative Names: Interferon Alpha/Beta Receptor 2, IFN-R-2, IFN-Alpha Binding Protein, IFN-Alpha/Beta Receptor 2, Interferon Alpha Binding Protein, Type I Interferon Receptor 2, IFNAR2, IFNABR, IFNARB - Molecular Weight
- 25.79 kDa
- UniProt
- P48551
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