IGFBP4 Protein (AA 22-258) (His tag)

Catalog No. ABIN1096870

Recombinant IGFBP4 protein expressed in Human Cells.

Quick Overview for IGFBP4 Protein (AA 22-258) (His tag) (ABIN1096870)

Target: IGFBP4 (Insulin-Like Growth Factor Binding Protein 4 (IGFBP4))

Protein Type: Recombinant

Origin: Human

Source: Human Cells

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 22-258

Purification tag / Conjugate: This IGFBP4 protein is labelled with His tag.

Purpose: Recombinant Human Insulin-Like Growth Factor-Binding Protein 4/IGFBP-4 (C-6His)

Sequence: DEAIHCPPCS EEKLARCRPP VGCEELVREP GCGCCATCAL GLGMPCGVYT PRCGSGLRCY PPRGVEKPLH TLMHGQGVCM ELAEIEAIQE SLQPSDKDEG DHPNNSFSPC SAHDRRCLQK HFAKIRDRST SGGKMKVNGA PREDARPVPQ GSCQSELHRA LERLAASQSR THEDLYIIPI PNCDRNGNFH PKQCHPALDG QRGKCWCVDR KTGVKLPGGL EPKGELDCHQ LADSFREVDH HHHHH

Characteristics: Recombinant Human Insulin-Like Growth Factor-Binding Protein 4/IGFBP-4 is produced by our mammalian expression system in human cells. The target protein is expressed with sequence (D22-E258) of Human IGFBP4 fused with a polyhistidine tag at the C-terminus.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.2.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: 4 °C/-20 °C/-80 °C

Storage Comment: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 3 months

Target Details

Target: IGFBP4 (Insulin-Like Growth Factor Binding Protein 4 (IGFBP4))

Alternative Name: igfbp-4

Sub Type: Fusionprotein

Background: IGFBP-4, whose full name is insulin-like growth factor-binding protein 4, is induced by forskolin and N6, O2’dibutyryl sdenosine 3’, or 5’-cyclic monophosphate. It contains IGFBP N-terminal domain and thyroglobulin type-1 domain, and can bind IGF2. The IGF-binding proteins can prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture.
Alternative Names: Insulin-Like Growth Factor-Binding Protein 4, IBP-4, IGF-Binding Protein 4, IGFBP-4, IGFBP4, IBP4

Molecular Weight: 27.01 kDa

UniProt: P22692

Pathways: WNT Signaling, Myometrial Relaxation and Contraction, Regulation of Carbohydrate Metabolic Process