PEG-G-CSF protein (His tag)
Quick Overview for PEG-G-CSF protein (His tag) (ABIN1097314)
Target
Protein Type
Origin
Source
Purity
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Purification tag / Conjugate
- His tag
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Purpose
- Recombinant Mouse Noggin/NOG (C-6His)
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Sequence
- QHYLHIRPAP SDNLPLVDLI EHPDPIFDPK EKDLNETLLR SLLGGHYDPG FMATSPPEDR PGGGGGPAGG AEDLAELDQL LRQRPSGAMP SEIKGLEFSE GLAQGKKQRL SKKLRRKLQM WLWSQTFCPV LYAWNDLGSR FWPRYVKVGS CFSKRSCSVP EGMVCKPSKS VHLTVLRWRC QRRGGQRCGW IPIQYPIISE CKCSCHHHHH H
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Characteristics
- Recombinant Human G-CSF produced in E. coli is a single. non-glycosylated. polypeptide chain containing 175 amino acids and having a molecular mass of 18.800 Dalton. PEG-GCSF is manufactured by attaching a 20.000 Dalton methoxypolyethylene glycol propionaldehyde (mPEG-ALD) to the N-terminal amino acid of G-CSF having a total molecular mass of 38.800 Dalton.
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Sterility
- 0.2 μm filtered
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Endotoxin Level
- Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test
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Comment
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Gene Name: CSF3 C17orf33 GCSF
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
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It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles. -
Buffer
- Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.
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Handling Advice
- Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
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Storage
- 4 °C/-20 °C/-80 °C
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Storage Comment
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Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- PEG-G-CSF
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Molecular Weight
- 23.9 kDa
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UniProt
- P97466
Target
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