BCAS2 Protein (AA 2-225) (His tag,T7 tag)

Catalog No. ABIN1097391

This Recombinant BCAS2 protein is produced in Escherichia coli (E. coli).

Quick Overview for BCAS2 Protein (AA 2-225) (His tag,T7 tag) (ABIN1097391)

Target: BCAS2 (Breast Carcinoma Amplified Sequence 2 (BCAS2))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 2-225

Purification tag / Conjugate: This BCAS2 protein is labelled with His tag,T7 tag.

Purpose: Recombinant Human BCAS2/DAM1 (C-6His, N-T7 tag)

Sequence: MASMTGGQQM GRGSMAGTGL VAGEVVVDAL PYFDQGYEAP GVREAAAALV EEETRRYRPT KNYLSYLTAP DYSAFETDIM RNEFERLAAR QPIELLSMKR YELPAPSSGQ KNDITAWQEC VNNSMAQLEH QAVRIENLEL MSQHGCNAWK VYNENLVHMI EHAQKELQKL RKHIQDLNWQ RKNMQLTAGS KLREMESNWV SLVSKNYEIE RTIVQLENEI YQIKQQHGEA NKENIRQDFL EHHHHHH

Characteristics: Recombinant Human Breast Carcinoma-Amplified Sequence 2/BCAS2 is produced by our E. coli expression system. The target protein is expressed with sequence (Ala2-Phe225) of Human BCAS2 fused with a 6His tag at the C-terminus.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM TrisHCl, 200 mM NaCl, 2 mM DTT, pH 8.0.

Preservative: Dithiothreitol (DTT)

Precaution of Use: This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: 4 °C/-20 °C/-80 °C

Storage Comment: Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 3 months

Target Details

Target: BCAS2 (Breast Carcinoma Amplified Sequence 2 (BCAS2))

Alternative Name: Breast Carcinoma-Amplified Sequence 2/BCAS2

Sub Type: Fusionprotein

Background: Breast Carcinoma-Amplified Sequence 2 (BCAS2) is a member of the SPF27 family. BCAS2 is a nuclear protein and widely expressed in many rtissues. BCAS2 is identified as being overexpressed in various breast cancer cell lines. BCAS2 is a component of the spliceosome, taking part in the removal of introns from mRNA precursors. BCAS2 interacts with estrogen receptor alpha and beta, thyroid hormone receptor beta, peroxisome proliferator-activated receptor gamma. BCAS2 functions as an ER co-activator and is capable of enhancing ER-mediated transcription.
Alternative Names: Pre-mRNA-Splicing Factor SPF27, Breast Carcinoma-Amplified Sequence 2, DNA Amplified in Mammary Carcinoma 1 Protein, Spliceosome-Associated Protein SPF 27, BCAS2, DAM1

Molecular Weight: 28.58 kDa

UniProt: O75934