SERPINA12 Protein (AA 21-414) (GST tag)

Catalog No. ABIN1097502

This Recombinant SERPINA12 protein is produced in Escherichia coli (E. coli).

Quick Overview for SERPINA12 Protein (AA 21-414) (GST tag) (ABIN1097502)

Target: SERPINA12 (serpin Peptidase Inhibitor, Clade A (Alpha-1 Antiproteinase, Antitrypsin), Member 12 (SERPINA12))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 21-414

Purification tag / Conjugate: This SERPINA12 protein is labelled with GST tag.

Purpose: Recombinant Human Serpin A12/Vaspin (N-GST)

Sequence: MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD LVPRGSLKPS FSPRNYKALS EVQGWKQRMA AKELARQNMD LGFKLLKKLA FYNPGRNIFL SPLSISTAFS MLCLGAQDST LDEIKQGFNF RKMPEKDLHE GFHYIIHELT QKTQDLKLSI GNTLFIDQRL QPQRKFLEDA KNFYSAETIL TNFQNLEMAQ KQINDFISQK THGKINNLIE NIDPGTVMLL ANYIFFRARW KHEFDPNVTK EEDFFLEKNS SVKVPMMFRS GIYQVGYDDK LSCTILEIPY QKNITAIFIL PDEGKLKHLE KGLQVDTFSR WKTLLSRRVV DVSVPRLHMT GTFDLKKTLS YIGVSKIFEE HGDLTKIAPH RSLKVGEAVH KAELKMDERG TEGAAGTGAQ TLPMETPLVV KIDKPYLLLI YSEKIPSVLF LGKIVNPIGK

Characteristics: Recombinant Human Serpin A12 is produced by our E. coli expression system. The target protein is expressed with sequence (Leu21-Lys414) of Human Serpin A12 fused with a GST tag at the N-terminus.

Sterility: 0.2 μm filtered

Endotoxin Level: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: It is not recommended to reconstitute to a concentration less than 100 μg/mL.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.

Buffer: Supplied as a 0.2 μm filtered solution of 50 mM TrisHCl, 160 mM NaCl, 0.2 mM PMSF, 1 mM DTT, 10 % Glycerine, pH 7.2 .

Preservative: Dithiothreitol (DTT)

Precaution of Use: This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Always centrifuge tubes before opening. Do not mix by vortex or pipetting.

Storage: -80 °C

Storage Comment: Store at < -20°C, stable for 6 months after receipt.
Please minimize freeze-thaw cycles.

Expiry Date: 6 months

Target Details

Target: SERPINA12 (serpin Peptidase Inhibitor, Clade A (Alpha-1 Antiproteinase, Antitrypsin), Member 12 (SERPINA12))

Alternative Name: Serpin A12

Sub Type: Fusionprotein

Background: Vaspin (Visceral Adipose-Specific SERPIN) is a newly described adipokine. Vaspin has three beta-sheets, nine alpha-helices, and one central loop, the structure is part of the set of distinctive features that are descriptive of Serpin family members. Vaspin is also a unique insulin sensitizing adipocytokine in obesity. A recent publication indicates that Vaspin mRNA expression in visceral fat is positively correlated with BMI and percent of body fat. and could be associated with parameters of obesity, insulin resistance, and glucose metabolism. These findings suggest a potential clinical use for Vaspin in ameliorating certain aberrations seen in the obesity metabolic syndrome.
Alternative Names: Serpin A12, OL-64, Visceral Adipose Tissue-Derived Serine Protease Inhibitor, Vaspin, Visceral Adipose-Specific Serpin, SERPINA12

Molecular Weight: 71.4 kDa

UniProt: Q8IW75